Metabolic Profile Discriminates and Predicts Arabidopsis Susceptibility to Virus under Field Conditions.
Metabolic Profile Discriminates and Predicts Arabidopsis Susceptibility to Virus under Field Conditions.
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DOI：
10.3390/metabo11040230
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发表时间：
2021-04-09
期刊：
影响因子：
4.1
通讯作者：
Schurdi-Levraud V
中科院分区：
文献类型：
--
作者：
Rubio B;Fernandez O;Cosson P;Berton T;Caballero M;Lion R;Roux F;Bergelson J;Gibon Y;Schurdi-Levraud V
植物病毒作为专性寄生物，改变宿主细胞的代谢。在自然环境中，每天变化的条件下，遗传多样的植物之间的病毒诱导的代谢反应的变异性的信息缺乏。为了破译植物-病毒相互作用在自然环境中的代谢景观，26和10加入拟南芥接种芜菁花叶病毒（TuMV），在两个田间试验超过2年。加入病毒积累，地上生物量，有针对性的和非靶向的代谢谱进行了测量。这些材料的表型从感病到抗病不等。感病和抗病材料接种后的代谢途径不同。易感基因型在感染后积累初级和次级代谢产物，以生长受阻为代价。21代谢签名显着积累抗性加入，而他们保持其增长模拟接种植物没有生物量惩罚。代谢内容被证明是歧视和高度预测接种拟南芥的易感性。这项研究首次描述了自然环境中植物-病毒相互作用的代谢景观及其与易感性的预测联系。它为植物-病毒相互作用提供了新的见解。在这种未驯化的物种和生态现实条件下，生长和抗性是一个永久的对话。
As obligatory parasites, plant viruses alter host cellular metabolism. There is a lack of information on the variability of virus-induced metabolic responses among genetically diverse plants in a natural context with daily changing conditions. To decipher the metabolic landscape of plant-virus interactions in a natural setting, twenty-six and ten accessions of Arabidopsis thaliana were inoculated with Turnip mosaic virus (TuMV), in two field experiments over 2 years. The accessions were measured for viral accumulation, above-ground biomass, targeted and untargeted metabolic profiles. The phenotypes of the accessions ranged from susceptibility to resistance. Susceptible and resistant accessions were shown to have different metabolic routes after inoculation. Susceptible genotypes accumulate primary and secondary metabolites upon infection, at the cost of hindered growth. Twenty-one metabolic signatures significantly accumulated in resistant accessions whereas they maintained their growth as mock-inoculated plants without biomass penalty. Metabolic content was demonstrated to discriminate and be highly predictive of the susceptibility of inoculated Arabidopsis. This study is the first to describe the metabolic landscape of plant-virus interactions in a natural setting and its predictive link to susceptibility. It provides new insights on plant-virus interactions. In this undomesticated species and in ecologically realistic conditions, growth and resistance are in a permanent conversation.
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影响因子：
3.7
作者：
Cuperlovic-Culf M;Wang L;Forseille L;Boyle K;Merkley N;Burton I;Fobert PR
通讯作者：
Fobert PR
影响因子：
2.9
作者：
Bantan-Polak, T;Kassai, M;Grant, KB
通讯作者：
Grant, KB
影响因子：
7.4
作者：
Fernandez-Calvino, Lourdes;Osorio, Sonia;Llave, Cesar
通讯作者：
Llave, Cesar
影响因子：
30.3
作者：
Chandran, Divya;Rickert, Joshua;Wildermuth, Mary C.
通讯作者：
Wildermuth, Mary C.
影响因子：
4.3
作者：
Biemelt, S;Sonnewald, U
通讯作者：
Sonnewald, U