Diamide insecticide resistance in transgenic Drosophila and Sf9-cells expressing a full-length diamondback moth ryanodine receptor carrying an I4790M mutation.
Diamide insecticide resistance in transgenic Drosophila and Sf9-cells expressing a full-length diamondback moth ryanodine receptor carrying an I4790M mutation.
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DOI：
10.1002/ps.6730
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发表时间：
2022-03
影响因子：
4.1
通讯作者：
Davies, Tg Emyr
中科院分区：
文献类型：
--
作者：
Richardson, Ewan;Homem, Rafael A.;Troczka, Bartlomiej J.;George, Christopher H.;Ebbinghaus-Kintscher, Ulrich;Williamson, Martin S.;Nauen, Ralf;Davies, Tg Emyr
关键词：
鳞翅目昆虫对二酰胺类杀虫剂的抗药性主要是由Ryanodine受体（RyR）上的氨基酸变化引起的。最近，两个新的靶位点突变，G4946 V和I4790 M，已经出现在种群的小菜蛾，小菜蛾，以及在其他鳞翅目物种，这两个突变已被证明经验，以减少二酰胺的功效。在这里，我们量化的影响I4790M突变对二酰胺激活的受体，相比，在G4946位点的改变。当将I4790 M引入昆虫源Sf 9细胞系中表达的小菜蛾RyR中时，发现I4790 M仅介导氯虫苯甲酰胺功效降低10倍（相比之下，G4946 E和G4946 V变体分别降低104倍和146倍），而在田间，它的存在与降低≥150倍有关。I4790M介导的对氟虫双胺的耐药性估计为>24倍。当将小菜蛾RyR的整个编码序列整合到黑腹果蝇中时，I4790M变体在3龄幼虫中赋予了对氯虫苯甲酰胺的约4.4倍抗性和对氟虫双酰胺的22倍抗性，证实其仅赋予了对双酰胺杀虫剂的中等水平的抗性。虽然I4790M替代似乎没有承担健身成本的苍蝇的繁殖能力，在非竞争性环境中进行评估时，它确实，但是，有潜在的重大影响的流动性在幼虫和成年阶段。I4790M仅赋予对二酰胺杀虫剂的中等水平的抗性，并可能赋予昆虫显著的适应性成本。PxRyR上的I4790M突变仅赋予对二酰胺杀虫剂的中等水平的抗性（与G4946E或G4946V相比），并可能赋予昆虫显著的适应性成本。版权所有© 2021作者。有害生物管理科学由John Wiley & Sons Ltd代表化学工业协会出版。
Resistance to diamide insecticides in Lepidoptera is known to be caused primarily by amino acid changes on the ryanodine receptor (RyR). Recently, two new target site mutations, G4946V and I4790M, have emerged in populations of diamondback moth, Plutella xylostella, as well as in other lepidopteran species, and both mutations have been shown empirically to decrease diamide efficacy. Here, we quantify the impact of the I4790M mutation on diamide activation of the receptor, as compared to alterations at the G4946 locus. I4790M when introduced into P. xylostella RyR expressed in an insect‐derived Sf9 cell line was found to mediate just a ten‐fold reduction in chlorantraniliprole efficacy (compared to 104‐ and 146‐fold reductions for the G4946E and G4946V variants, respectively), whilst in the field its presence is associated with a ≥150‐fold reduction. I4790M‐mediated resistance to flubendiamide was estimated to be >24‐fold. When the entire coding sequence of P. xylostella RyR was integrated into Drosophila melanogaster, the I4790M variant conferred ~4.4‐fold resistance to chlorantraniliprole and 22‐fold resistance to flubendiamide in the 3rd instar larvae, confirming that it imparts only a moderate level of resistance to diamide insecticides. Although the I4790M substitution appears to bear no fitness costs in terms of the flies' reproductive capacity, when assessed in a noncompetitive environment, it does, however, have potentially major impacts on mobility at both the larval and adult stages. I4790M imparts only a moderate level of resistance to diamide insecticides and potentially confers significant fitness costs to the insect. The I4790M mutation on PxRyR imparts only a moderate level of resistance to diamide insecticides (as compared to G4946E or G4946V) and potentially confers significant fitness costs to the insect. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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影响因子：
7.7
作者：
Lee PT;Zirin J;Kanca O;Lin WW;Schulze KL;Li-Kroeger D;Tao R;Devereaux C;Hu Y;Chung V;Fang Y;He Y;Pan H;Ge M;Zuo Z;Housden BE;Mohr SE;Yamamoto S;Levis RW;Spradling AC;Perrimon N;Bellen HJ
通讯作者：
Bellen HJ
影响因子：
4.6
作者：
Troczka BJ;Williams AJ;Williamson MS;Field LM;Lüemmen P;Davies TG
通讯作者：
Davies TG
影响因子：
2.4
作者：
Fukada, Megumi;Itagaki, Yuki;Sonoda, Shoji
通讯作者：
Sonoda, Shoji
影响因子：
3.8
作者：
Troczka, Bartek;Zimmer, Christoph T.;Nauen, Ralf
通讯作者：
Nauen, Ralf
鳞翅目动物中发生的多个靶位点突变赋予对二酰胺杀虫剂的抗性
影响因子：
3.8
作者：
Huang, Jing-Mei;Rao, Cong;Wu, Shun-Fan
通讯作者：
Wu, Shun-Fan