Comparative analysis of various clinical specimens in detection of SARS-CoV-2 using rRT-PCR in new and follow up cases of COVID-19 infection: Quest for the best choice.
Comparative analysis of various clinical specimens in detection of SARS-CoV-2 using rRT-PCR in new and follow up cases of COVID-19 infection: Quest for the best choice.
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DOI：
10.1371/journal.pone.0249408
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Nagarkar NM
中科院分区：
文献类型：
--
作者：
Sharma K;Aggarwala P;Gandhi D;Mathias A;Singh P;Sharma S;Negi SS;Bhargava A;Das P;Gaikwad U;Wankhede A;Behra A;Nagarkar NM
在基于实时逆转录聚合酶链反应（rRT-PCR）的新型冠状病毒（nCoV）病（COVID-19）诊断中，合适的标本至关重要。因此，评估各种多样化临床标本在COVID-19诊断和随访中的诊断效用是有针对性的。采用rRT-PCR方法对130例COVID-19症状患者治疗前的924份初始标本和随机抽取的15例患者的665份随访标本进行评估，包括等量的鼻咽拭子（NPS）、口咽拭子（OPS）、NPS和OPS联合拭子（联合拭子）、痰、血浆、血清和尿液。人口统计学分析显示，男性（86人）比女性（44人）多2倍（p = 0.00001）。联合拭子阳性率为100%，其次为NPS（91.5%）、OPS（72.3%）、痰液（63%），尿液、血浆和血清标本中未检出新型冠状病毒。靶基因E、ORF1b和RdRP的最低周期阈值（Ct）分别为10.56、10.14和12.26，其平均Ct值在联合拭子中最低，表明联合拭子在所有标本类型中病毒载量较高。对665份随访多品种标本的分析也显示，在所有标本类型中，联合拭子是最后一个呈阴性的标本，平均在治疗后6.6天（4-10天），ORF1b的Ct值最低（15.48），平均（29.96），分别表明后鼻咽道是新型冠状病毒的主要感染部位，病毒载量高。建议将联合拭子作为更合适的标本用于rRT-PCR诊断和监测COVID-19治疗，以评估病毒清除情况，帮助医生在出院前做出循证决策。在全球范围内实施联合拭子肯定有助于管理和控制大流行，因为这是当前的需要。
An appropriate specimen is of paramount importance in Real Time reverse transcription-polymerase chain reaction (rRT-PCR) based diagnosis of novel coronavirus (nCoV) disease (COVID-19). Thus, it’s pertinent to evaluate various diversified clinical specimens’ diagnostic utility in both diagnosis and follow-up of COVID-19. A total of 924 initial specimens from 130 COVID-19 symptomatic cases before initiation of treatment and 665 follow up specimens from 15 randomly selected cases comprising of equal number of nasopharyngeal swab (NPS), oropharyngeal swab (OPS), combined NPS and OPS (Combined swab), sputum, plasma, serum and urine were evaluated by rRT-PCR. Demographic analysis showed males (86) twice more affected by COVID-19 than females (44) (p = 0.00001). Combined swabs showed a positivity rate of 100% followed by NPS (91.5%), OPS (72.3%), sputum (63%), while nCoV was found undetected in urine, plasma and serum specimens. The lowest cycle threshold (Ct) values of targeted genes E, ORF1b and RdRP are 10.56, 10.14 and 12.26 respectively and their lowest average Ct values were found in combined swab which indicates high viral load in combined swab among all other specimen types. Analysis of 665 follow-up multi-varied specimens also showed combined swab as the last specimen among all specimen types to become negative, after an average 6.6 (range 4–10) days post-treatment, having lowest (15.48) and average (29.96) Ct values of ORF1b respectively indicating posterior nasopharyngeal tract as primary nCoV afflicted site with high viral load. The combined swab may be recommended as a more appropriate specimen for both diagnosis and monitoring of COVID-19 treatment by rRT-PCR for assessing virus clearance to help physicians in taking evidence-based decision before discharging patients. Implementing combined swabs globally will definitely help in management and control of the pandemic, as it is the need of the hour.
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DOI：
10.1007/s42399-020-00341-w
发表时间：
2020-01-01
期刊：
SN comprehensive clinical medicine
影响因子：
--
作者：
Bwire, George M
通讯作者：
Bwire, George M
影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
影响因子：
3.9
作者：
Czumbel, Laszlo Mark;Kiss, Szabolcs;Varga, Gabor
通讯作者：
Varga, Gabor
影响因子：
7.4
作者：
Tong Y;Bao A;Chen H;Huang J;Lv Z;Feng L;Cheng Y;Wang Y;Bai L;Rao W;Zheng H;Wu Z;Qiao B;Zhao Z;Wang H;Li Y
通讯作者：
Li Y
使用 RT-PCR 检测拭子类型和采集时间对 SARS-COV-2 检测的价值
影响因子：
3.1
作者：
Liu, Min;Li, Qianyuan;Luo, Chunhua
通讯作者：
Luo, Chunhua