Necessity for detection of SARS-CoV-2 RNA in multiple types of specimens for the discharge of the patients with COVID-19.
Necessity for detection of SARS-CoV-2 RNA in multiple types of specimens for the discharge of the patients with COVID-19.
复制标题
DOI：
10.1186/s12967-020-02580-w
复制
发表时间：
2020-11-02
影响因子：
7.4
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Tong Y;Bao A;Chen H;Huang J;Lv Z;Feng L;Cheng Y;Wang Y;Bai L;Rao W;Zheng H;Wu Z;Qiao B;Zhao Z;Wang H;Li Y
一些有或没有发烧或干咳等临床症状的COVID-19患者出院后再次检测到SARS-CoV-2 RNA阳性。选择1008例实时荧光定量PCR（RT-qPCR）鼻咽拭子和口咽拭子混合标本检测出SARS-CoV-2 RNA阳性的1008例重症COVID-19患者，在住院期间对12种标本进行RT-qPCR监测SARS-CoV-2 RNA。全部20例出院的COVID-19病例在隔离期解除前，对7种标本进行RT-qPCR检测隔离期SARS-CoV-2 RNA。入组的1008例重症患者中，鼻咽拭子标本SARS-CoV-2 RNA阳性率最高（71.06%），其次是肺泡灌洗液（66.67%）、口咽拭子（30.77%）、痰（28.53%）、尿液（16.30%）、血液（12.5%）、粪便本研究中其他类型标本均未检出SARS-CoV-2 RNA（12.21%）、肛拭子（11.22%）和角膜分泌物（2.99%）。隔离期间出院的20例病例中，SARS-CoV-2 RNA阳性率为30%（6/20）：出院后第8天和第9天痰液阳性分别2例，出院后第6天鼻咽拭子阳性1例，出院后第8天肛拭子阳性1例，3份标本（鼻咽拭子）阳性1例出院后第四天同时进行拭子、口咽拭子和痰）检测，出院患者的粪便、尿液和血液等其他标本中均未检测到SARS-CoV-2 RNA阳性。应在多个标本中检测SARS-CoV-2 RNA，如鼻咽拭子、口咽拭子、痰液，必要时应在患者考虑临床治愈出院时和解除隔离期前同时进行粪便和肛拭子标本检测。
The SARS-CoV-2 RNA was detected positive again after discharged from hospital in some COVID-19 patients, with or without clinical symptoms such as fever or dry cough. 1008 severe COVID-19 patients, with SARS-CoV-2 RNA positive detected with the mixed specimen of nasopharyngeal swab and oropharyngeal swab by real-time fluorescence quantitative PCR (RT-qPCR), were selected to monitor SARS-CoV-2 RNA with the 12 types of specimens by RT-qPCR during hospitalization. All of 20 discharged cases with COVID-19 were selected to detect SARS-CoV-2 RNA in isolation period with 7 types of specimens by RT-qPCR before releasing the isolation period. Of the enrolled 1008 severe patients, the nasopharyngeal swab specimens showed the highest positive rate of SARS-CoV-2 RNA (71.06%), followed by alveolar lavage fluid (66.67%), oropharyngeal swab (30.77%), sputum (28.53%), urine (16.30%), blood (12.5%), stool (12.21%), anal swab (11.22%) and corneal secretion (2.99%), and SARS-CoV-2 RNA couldn’t be detected in other types of specimen in this study. Of the 20 discharged cases during the isolation period, the positive rate of SARS-CoV-2 RNA was 30% (6/20): 2 cases were positive in sputum at the eighth and ninth day after discharge, respectively, 1 case was positive in nasopharynx swab at the sixth day after discharge, 1 case was positive in anal swab at the eighth day after discharge, and 1 case was positive in 3 specimens (nasopharynx swab, oropharynx swab and sputum) simultaneously at the fourth day after discharge, and no positive SARS-CoV-2 RNA was detected in other specimens including stool, urine and blood at the discharged patients. SARS-CoV-2 RNA should be detected in multiple specimens, such as nasopharynx swab, oropharynx swab, sputum, and if necessary, stool and anal swab specimens should be performed simultaneously at discharge when the patients were considered for clinical cure and before releasing the isolation period.
登录
查看更多内容
影响因子：
12.7
作者：
Peng, Liang;Liu, Jing;Gao, Zhiliang
通讯作者：
Gao, Zhiliang
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
12.7
作者：
Cao, Hong;Ruan, Lei;Liao, Wenhui
通讯作者：
Liao, Wenhui
血液中可检测到的 2019-nCoV 病毒 RNA 是进一步临床严重程度的有力指标
影响因子：
13.2
作者：
Chen, Weilie;Lan, Yun;Tang, Xiaoping
通讯作者：
Tang, Xiaoping
DOI：
10.1016/j.nano.2019.03.006
发表时间：
2019-06-01
影响因子：
5.4
作者：
Aquino-Jarquin, Guillermo
通讯作者：
Aquino-Jarquin, Guillermo