Comprehensive transcriptomics, proteomics, and metabolomics analyses of the mechanisms regulating tiller production in low-tillering wheat
Comprehensive transcriptomics, proteomics, and metabolomics analyses of the mechanisms regulating tiller production in low-tillering wheat
复制标题
低分蘖小麦分蘖调控机制的综合转录组学、蛋白质组学和代谢组学分析
DOI：
10.1007/s00122-019-03345-w
复制
发表时间：
2019-04
影响因子：
5.4
通讯作者：
Liu Yaxi
中科院分区：
文献类型：
--
作者：
Wang Zhiqiang;Shi Haoran;Yu Shifan;Zhou Wanlin;Li Jing;Liu Shihang;Deng Mei;Ma Jian;Wei Yuming;Zheng Youliang;Liu Yaxi
KeymessageTiller的发展在低分蘖小麦相关的几个差异表达的基因，蛋白质和代谢产物，确定了一个综合的组学方法相结合的转录组分析，iTRAQ，和HPLC-MS多个NILs.AbstractTillering是一个重要的方面，植物形态，影响穗数，从而有助于最终作物产量。然而，在低分蘖小麦分蘖生产的抑制机制是很差的特点。为了研究小麦生物学的这一方面，开发了两对近等基因系，并使用整合组学方法结合转录组学分析、用于相对和绝对定量的同量异位素标签以及高效液相色谱-质谱来比较小麦样品中由等位基因atQltn.sicau-2D引起的自由分蘖和低分蘖。总的来说，474个基因，166个蛋白质和28个代谢产物被鉴定为分蘖相关的差异表达基因，蛋白质和代谢产物（DEG，DEP和DEM，分别）。功能分析表明，DEGs/DEPs/DEMs的丰度与木质素和纤维素代谢、细胞分裂、细胞周期进程和甘油磷脂代谢有关; GRAS、GRF和REV 3个转录因子家族可能与低分蘖小麦分蘖减少有关。这些发现有助于提高我们对低分蘖小麦品种分蘖发育抑制机制的理解。
Key messageTiller development in low-tillering wheat is related to several differentially expressed genes, proteins, and metabolites, as determined by an integrated omics approach combining transcriptome analysis, iTRAQ, andHPLC-MSon multiple NILs.AbstractTillering is an important aspect of plant morphology that affects spike number, thereby contributing to the final crop yield. However, the mechanisms inhibiting tiller production in low-tillering wheat are poorly characterized. To investigate this aspect of wheat biology, two pairs of near-isogenic lines were developed, and an integrated omics approach combining transcriptome analysis, isobaric tags for relative and absolute quantification, and high-performance liquid chromatography-mass spectrometry were used to compare the free-tillering and low-tillering caused by an allele atQltn.sicau-2Din wheat samples. Overall, 474 genes, 166 proteins, and 28 metabolites were identified as tillering-associated differentially expressed genes, proteins, and metabolites (DEGs, DEPs, and DEMs, respectively). Functional analysis indicated that the abundance of DEGs/DEPs/DEMs was related to lignin and cellulose metabolism, cell division, cell cycle processes, and glycerophospholipid metabolism; three transcription factor families,GRAS,GRF, andREV, might be related to the decrease in tillering in low-tillering wheat. These findings contribute to improve our understanding of the mechanisms responsible for the inhibition of tiller development in low-tillering wheat cultivars.
登录
查看更多内容
基于LC-MS的大戟治疗大鼠毒性生物标志物血浆代谢组学研究
影响因子：
1.8
作者：
Wang Yingfeng;Man Hongxue;Gao Jian;Liu Xinfeng;Ren Xiaolei;Chen Jianxin;Zhang Jiayu;Gao Kuo;Li Zhongfeng;Zhao Baosheng
通讯作者：
Zhao Baosheng
影响因子：
5.6
作者：
Moeller C;Evers JB;Rebetzke G
通讯作者：
Rebetzke G
DOI：
10.1105/tpc.010146
发表时间：
2001-09
期刊：
The Plant Cell Online
影响因子：
--
作者：
C. Helliwell;A. N. Chin-Atkins;I. Wilson;R. Chapple;E. Dennis;A. Chaudhury
通讯作者：
C. Helliwell;A. N. Chin-Atkins;I. Wilson;R. Chapple;E. Dennis;A. Chaudhury
影响因子：
7.4
作者：
Kuijt, Suzanne J. H.;Greco, Raffaella;Ouwerkerk, Pieter B. F.
通讯作者：
Ouwerkerk, Pieter B. F.
影响因子：
7.4
作者：
Liu, Jie;Cheng, Xiliu;Sun, Jiaqiang
通讯作者：
Sun, Jiaqiang