Differential Effects of Transition Metals on Growth and Metal Uptake for Two Distinct Lactobacillus Species.
Differential Effects of Transition Metals on Growth and Metal Uptake for Two Distinct Lactobacillus Species.
复制标题
过渡金属对两种不同乳酸菌生长和金属吸收的不同影响。
DOI：
10.1128/spectrum.01006-21
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发表时间：
2022-02-23
影响因子：
3.7
通讯作者：
Zastrow ML
中科院分区：
文献类型：
--
作者：
Huynh U;Qiao M;King J;Trinh B;Valdez J;Haq M;Zastrow ML
乳杆菌属是革兰氏阳性细菌的一个属，并且包含将糖转化为乳酸的乳酸菌群的主要部分。在肠道微生物群中发现的乳杆菌物种被认为对人类健康有益，并且常用于益生菌制剂中，但它们的分子功能仍然不清楚。微生物需要金属离子来生长和发挥功能，并且必须从周围环境中获得它们。因此，乳酸杆菌需要与其他肠道微生物竞争这些营养素，尽管它们的金属需求还不清楚。事实上，微生物群中乳酸杆菌的丰度经常受到锌，锰和铁等必需金属的饮食摄入的影响，但很少有研究调查金属，特别是锌，在乳酸杆菌物种的生理和代谢中的作用。在这里，我们研究了金属的吸收，通过量化总细胞的金属含量，并比较过渡金属如何影响两种不同的乳杆菌，植物乳杆菌ATCC 14917和嗜酸乳杆菌ATCC 4356的生长。当生长在丰富或金属有限的介质中，这两个物种采取了更多的锰，锌和铁相比，其他过渡金属测量。在这些物种的生长动力学中观察到不同的锌，锰和铁依赖模式，而每种金属的某些水平促进了两种乳杆菌属物种的生长动力学，其影响显着依赖于培养基和生长条件。胃肠道含有数万亿的微生物，对人类健康至关重要。乳酸杆菌被认为是有益的微生物群成员，经常用于益生菌，但它们的分子功能，特别是那些依赖金属的功能，仍然很难定义。微生物群中乳酸杆菌的减少经常受到锰，锌和铁等必需金属的饮食摄入的影响，但结果是复杂的，有时是矛盾的，并且难以预测。鉴于微生物群组成的变化与疾病和感染有关，因此非常需要了解宿主的饮食和代谢将如何影响微生物群。我们研究的意义在于深入了解金属如何明显影响单个乳酸菌物种，这可能导致新的治疗方法和改善医疗。生长动力学和金属含量的量化突出了不同的物种如何对不同的金属可用性做出不同的反应，并为未来的分子和机理研究提供了基础。
Lactobacillus is a genus of Gram-positive bacteria and comprises a major part of the lactic acid bacteria group that converts sugars to lactic acid. Lactobacillus species found in the gut microbiota are considered beneficial to human health and commonly used in probiotic formulations, but their molecular functions remain poorly defined. Microbes require metal ions for growth and function and must acquire them from the surrounding environment. Therefore, lactobacilli need to compete with other gut microbes for these nutrients, although their metal requirements are not well-understood. Indeed, the abundance of lactobacilli in the microbiota is frequently affected by dietary intake of essential metals like zinc, manganese, and iron, but few studies have investigated the role of metals, especially zinc, in the physiology and metabolism of Lactobacillus species. Here, we investigated metal uptake by quantifying total cellular metal contents and compared how transition metals affect the growth of two distinct Lactobacillus species, Lactobacillus plantarum ATCC 14917 and Lactobacillus acidophilus ATCC 4356. When grown in rich or metal-limited medium, both species took up more manganese, zinc, and iron compared with other transition metals measured. Distinct zinc-, manganese- and iron-dependent patterns were observed in the growth kinetics for these species and while certain levels of each metal promoted the growth kinetics of both Lactobacillus species, the effects depend significantly on the culture medium and growth conditions. IMPORTANCE The gastrointestinal tract contains trillions of microorganisms, which are central to human health. Lactobacilli are considered beneficial microbiota members and are often used in probiotics, but their molecular functions, and especially those which are metal-dependent, remain poorly defined. Abundance of lactobacilli in the microbiota is frequently affected by dietary intake of essential metals like manganese, zinc, and iron, but results are complex, sometimes contradictory, and poorly predictable. There is a significant need to understand how host diet and metabolism will affect the microbiota, given that changes in microbiota composition are linked with disease and infection. The significance of our research is in gaining insight to how metals distinctly affect individual Lactobacillus species, which could lead to novel therapeutics and improved medical treatment. Growth kinetics and quantification of metal contents highlights how distinct species can respond differently to varied metal availability and provide a foundation for future molecular and mechanistic studies.
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影响因子：
2.1
作者：
ARCHIBALD, F
通讯作者：
ARCHIBALD, F
影响因子：
3.2
作者：
ARCHIBALD, FS;FRIDOVICH, I
通讯作者：
FRIDOVICH, I
影响因子：
2.1
作者：
Boehmer, Nico;Koenig, Saskia;Fischer, Lutz
通讯作者：
Fischer, Lutz
DOI：
10.1111/j.1365-2672.1960.tb00188.x
发表时间：
1960-01-01
期刊：
JOUR APPL BACT
影响因子：
--
作者：
DE MAN, J. C.;ROGOSA, M.;SHARPE, M. ELIZABETH
通讯作者：
SHARPE, M. ELIZABETH
影响因子：
3.3
作者：
Celebioglu, Hasan Ufuk;Olesen, Sita Vaag;Svensson, Birte
通讯作者：
Svensson, Birte