Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
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DOI：
10.1021/cr900033p
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发表时间：
2009-09
期刊：
影响因子：
62.1
通讯作者：
Iwahara, Junji
中科院分区：
文献类型：
--
作者：
Clore, G. Marius;Iwahara, Junji
在物理化学水平上了解生物大分子及其复合物的功能需要了解其结构和动力学。传统的生物物理技术，例如晶体学和核磁共振，已经在原子水平上产生了关于高密度静态的极其详细的结构信息。 1 在大分子的能量景观表示的背景下，高密度态位于相对粗糙的自由能超曲面的全局自由能最小区域内。 2-5 然而，对于人口稀少、自由能较高的状态，我们知之甚少，而这些状态是无法被确定的。
Understanding the function of biological macromolecules and their complexes at the physicochemical level requires knowledge of both their structure and dynamics. Conventional biophysical techniques, such as crystallography and NMR, have yielded incredibly detailed structural information at the atomic level on highly populated static states. 1 In the context of the energy landscape representation of macromolecules, highly populated states are located within the global free energy minimum region of a relatively rough free energy hypersurface. 2-5 Much less is known, however, about lowly populated, higher free energy states which cannot be
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DOI：
10.1073/pnas.0407146102
发表时间：
2005-02-01
影响因子：
11.1
作者：
Bertoncini, CW;Jung, YS;Zweckstetter, M
通讯作者：
Zweckstetter, M
影响因子：
15
作者：
Balayssac, Stephane;Bertini, Ivano;Piccioli, Mario
通讯作者：
Piccioli, Mario
影响因子：
2.2
作者：
Bermel, W;Bertini, I;Pierattelli, R
通讯作者：
Pierattelli, R
影响因子：
5.6
作者：
BERG, OG;VONHIPPEL, PH
通讯作者：
VONHIPPEL, PH
影响因子：
2.9
作者：
Baker, KA;Hilty, C;Choe, S
通讯作者：
Choe, S