Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances.
Environmental DNA (eDNA) detects the invasive rusty crayfish Orconectes rusticus at low abundances.
复制标题
DOI：
10.1111/1365-2664.12621
复制
发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Lodge DM
中科院分区：
文献类型：
--
作者：
Dougherty MM;Larson ER;Renshaw MA;Gantz CA;Egan SP;Erickson DM;Lodge DM
及早发现对于具有成本效益的控制和根除入侵物种来说是非常宝贵的，但许多传统的抽样技术在入侵过程开始时发现种群丰度低的情况下是无效的。环境DNA(EDNA)是一种很有前途的敏感工具，可用于早期检测一些入侵物种，但它对许多分类群体和栖息地类型的有效性尚未得到评估。我们评估了EDNA检测入侵的锈色小龙虾Orconectes Rusticus的能力，并反映了它在美国中西部北部内陆湖泊中的相对丰度模式。我们将传统的诱饵诱捕作为衡量小龙虾相对丰度的指标，并将其与EDNA的水样配对，这些水样在实验室用qPCR方法进行了分析。我们以相对丰度和地点特征为协变量，对稻纵卷叶蜂的检测概率进行了建模，并检验了斜纹夜蛾拷贝数与相对丰度之间的关系。我们在所有通过诱捕收集到这种物种的湖泊中都检测到了O.rusticus Edna，直到相对丰度很低，以及在两个捕获量为零的湖泊中。该物种的相对丰度和湖水的清晰度可以很好地预测该物种的检测概率。然而，埃德纳拷贝数与诱捕量估计的俄罗斯对虾相对丰度之间的一致性较差。合成与应用。我们的研究展示了EDNA监测小龙虾入侵的现场和实验室方案，统计模型的结果为其他地区和系统的研究人员提供了抽样努力和检测概率的指导。我们建议将Edna作为监测入侵或危险的小龙虾和其他底栖节肢动物的工具。我们的研究展示了EDNA监测小龙虾入侵的现场和实验室方案，统计模型的结果为其他地区和系统的研究人员提供了抽样努力和检测概率的指导。我们建议将Edna作为监测入侵或危险的小龙虾和其他底栖节肢动物的工具。
Early detection is invaluable for the cost‐effective control and eradication of invasive species, yet many traditional sampling techniques are ineffective at the low population abundances found at the onset of the invasion process. Environmental DNA (eDNA) is a promising and sensitive tool for early detection of some invasive species, but its efficacy has not yet been evaluated for many taxonomic groups and habitat types. We evaluated the ability of eDNA to detect the invasive rusty crayfish Orconectes rusticus and to reflect patterns of its relative abundance, in upper Midwest, USA, inland lakes. We paired conventional baited trapping as a measure of crayfish relative abundance with water samples for eDNA, which were analysed in the laboratory with a qPCR assay. We modelled detection probability for O. rusticus eDNA using relative abundance and site characteristics as covariates and also tested the relationship between eDNA copy number and O. rusticus relative abundance. We detected O. rusticus eDNA in all lakes where this species was collected by trapping, down to low relative abundances, as well as in two lakes where trap catch was zero. Detection probability of O. rusticus eDNA was well predicted by relative abundance of this species and lake water clarity. However, there was poor correspondence between eDNA copy number and O. rusticus relative abundance estimated by trap catches. Synthesis and applications. Our study demonstrates a field and laboratory protocol for eDNA monitoring of crayfish invasions, with results of statistical models that provide guidance of sampling effort and detection probabilities for researchers in other regions and systems. We propose eDNA be included as a tool in surveillance for invasive or imperilled crayfishes and other benthic arthropods. Our study demonstrates a field and laboratory protocol for eDNA monitoring of crayfish invasions, with results of statistical models that provide guidance of sampling effort and detection probabilities for researchers in other regions and systems. We propose eDNA be included as a tool in surveillance for invasive or imperilled crayfishes and other benthic arthropods.
登录
查看更多内容
调查环境 DNA (eDNA) 在海洋哺乳动物遗传监测中的潜在用途。
影响因子：
3.7
作者：
Foote AD;Thomsen PF;Sveegaard S;Wahlberg M;Kielgast J;Kyhn LA;Salling AB;Galatius A;Orlando L;Gilbert MT
通讯作者：
Gilbert MT
影响因子：
1.1
作者：
CAPELLI, GM;MAGNUSON, JJ
通讯作者：
MAGNUSON, JJ
影响因子：
4.8
作者：
Clark, JS
通讯作者：
Clark, JS
影响因子：
5.7
作者：
Dejean, Tony;Valentini, Alice;Miaud, Claude
通讯作者：
Miaud, Claude
影响因子：
2.2
作者：
Dopazo, Silvia N.;Corkum, Lynda D.;Mandrak, Nicholas E.
通讯作者：
Mandrak, Nicholas E.