Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study.
Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study.
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DOI：
10.3389/fendo.2020.571357
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发表时间：
2020
影响因子：
5.2
通讯作者：
Atkin SL
中科院分区：
文献类型：
--
作者：
Butler AE;Ramachandran V;Cunningham TK;David R;Gooderham NJ;Benurwar M;Dargham SR;Hayat S;Sathyapalan T;Najafi-Shoushtari SH;Atkin SL
小非编码microRNA(MiRNA)在多囊卵巢综合征(PCOS)中具有不同于非PCOS妇女的调节功能。然而，对非胰岛素抵抗(IR)的多囊卵巢综合征(PCOS)患者miRNA的表达知之甚少。用定量聚合酶链式反应(QPCR)检测了24例非肥胖BMI和年龄匹配的PCOS妇女和24例对照妇女的循环miRNAs。使用miRNA数据集来确定miRNA水平。PCOS患者表现出更高的游离雄激素指数(FAI)和抗苗勒氏激素(AMH)，但IR没有差别。在检测到的177个循环miRNAs中，有4个miRNAs(miR-1260a、miR-18b-5p、miR-424-5p和miR let-7b-3p)在对照组和PCOS妇女中不同，它们通过了假发现率(FDR)。在PCOS中，miRNA let-7b-3p与AMH相关(p<；0.05)。当两组合并时，miR-1260a与FAI相关，let-7b-3p与体重指数(BMI)相关(p<；0.05)。这与雄激素水平没有相关性。独创性途径分析显示，报道的前10个miRNAs中有9个与炎症途径有关。当IR在PCOS和对照组之间没有差异时，只有四个miRNA有显著差异，这表明IR可能是许多已报道的miRNA变化的驱动因素。LET-7b-3p与PCOS的AMH、BMI呈正相关，而miR-1260a与FAI呈正相关。然而，雄激素水平对循环miRNA图谱没有影响。表达的miRNAs与涉及肿瘤坏死因子和白介素6的炎症途径有关。
Small noncoding microRNA (miRNA) have regulatory functions in polycystic ovary syndrome (PCOS) that differ to those in women without PCOS. However, little is known about miRNA expression in women with PCOS who are not insulin resistant (IR). Circulating miRNAs were measured using quantitative polymerase chain reaction (qPCR) in 24 non-obese BMI and age matched women with PCOS and 24 control women. A miRNA data set was used to determine miRNA levels. Women with PCOS showed a higher free androgen index (FAI) and anti-mullerian hormone (AMH) but IR did not differ. Four miRNAs (miR-1260a, miR-18b-5p, miR-424-5p, and miR let-7b-3p) differed between control and PCOS women that passed the false discovery rate (FDR) out of a total of 177 circulating miRNAs that were detected. MiRNA let-7b-3p correlated with AMH in PCOS (p < 0.05). When the groups were combined, miR-1260a correlated with FAI and let-7b-3p correlated with body mass index (BMI) (p < 0.05). There was no correlation to androgen levels. Ingenuity pathway analysis showed that nine of the top 10 miRNAs reported were associated with inflammatory pathways. When IR did not differ between PCOS and control women, only four miRNA differed significantly suggesting that IR may be a driver for many of the miRNA changes reported. Let-7b-3p was related to AMH in PCOS, and to BMI as a group, whilst miR-1260a correlated with FAI. Androgen levels, however, had no effect upon circulating miRNA profiles. The expressed miRNAs were associated with the inflammatory pathway involving TNF and IL6.
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影响因子：
64.5
作者：
Kleaveland B;Shi CY;Stefano J;Bartel DP
通讯作者：
Bartel DP
影响因子：
4.6
作者：
Butler, Alexandra E.;Ramachandran, Vimal;Atkin, Stephen L.
通讯作者：
Atkin, Stephen L.
影响因子：
2
作者：
BIRKETT, MA;DAY, SJ
通讯作者：
DAY, SJ
影响因子：
6.1
作者：
Fauser, BCJM;Chang, J;Lobo, R
通讯作者：
Lobo, R
miRNA-93抑制GLUT4，并在多囊卵巢综合征患者和具有胰岛素抵抗的女性的脂肪组织中过表达。
影响因子：
7.7
作者：
Chen YH;Heneidi S;Lee JM;Layman LC;Stepp DW;Gamboa GM;Chen BS;Chazenbalk G;Azziz R
通讯作者：
Azziz R