Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 2. Label-free relative quantitative proteomics.
Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 2. Label-free relative quantitative proteomics.
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DOI：
10.1039/c6mb00290k
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发表时间：
2016-08-16
影响因子：
--
通讯作者：
Zadoks RN
中科院分区：
文献类型：
--
作者：
Mudaliar M;Tassi R;Thomas FC;McNeilly TN;Weidt SK;McLaughlin M;Wilson D;Burchmore R;Herzyk P;Eckersall PD;Zadoks RN
牛乳的纵向蛋白质组学分析显示，在用链球菌乳房内攻击后，奶牛随时间的变化一致。乳腺炎，即乳腺的炎症，是西方世界奶牛最常见和最昂贵的疾病。它主要由细菌引起，链球菌是最普遍的病原体之一。为了表征链球菌乳腺炎期间的蛋白质组，使用实验诱导的乳房内感染模型。采用无标记相对定量蛋白质组学方法对6头奶牛在6个时间点的乳清样品进行处理。这种蛋白质组学分析补充了临床、细菌学和免疫学研究以及相同挑战模型的肽组学和代谢组学分析。共鉴定了2552种非冗余牛肽，并从这些肽中定量了570种牛蛋白。层次聚类分析和主成分分析显示，感染阶段的结果有明显的聚类，感染前和消退阶段（攻毒后0和312 h）、早期感染阶段（攻毒后36和42 h）和晚期感染阶段（攻毒后57和81 h）之间具有相似性。免疫途径分析鉴定了急性期蛋白途径在感染过程中的上调，基于差异表达分析，不同急性期蛋白在不同时间点占优势。抗菌肽，特别是cathelicidins和肽聚糖识别蛋白，上调在所有时间点后的挑战，并在57小时达到峰值，这正好与平均细菌计数减少10 000倍。临床，细菌学，免疫学和定量蛋白质组学和其他组学数据的整合提供了一个更详细的系统水平的视图主机乳腺炎的反应比以前已经实现。
Longitudinal proteomic analysis of bovine milk shows consistent changes over time across cows after intramammary challenge with Streptococcus uberis. Mastitis, inflammation of the mammary gland, is the most common and costly disease of dairy cattle in the western world. It is primarily caused by bacteria, with Streptococcus uberis as one of the most prevalent causative agents. To characterize the proteome during Streptococcus uberis mastitis, an experimentally induced model of intramammary infection was used. Milk whey samples obtained from 6 cows at 6 time points were processed using label-free relative quantitative proteomics. This proteomic analysis complements clinical, bacteriological and immunological studies as well as peptidomic and metabolomic analysis of the same challenge model. A total of 2552 non-redundant bovine peptides were identified, and from these, 570 bovine proteins were quantified. Hierarchical cluster analysis and principal component analysis showed clear clustering of results by stage of infection, with similarities between pre-infection and resolution stages (0 and 312 h post challenge), early infection stages (36 and 42 h post challenge) and late infection stages (57 and 81 h post challenge). Ingenuity pathway analysis identified upregulation of acute phase protein pathways over the course of infection, with dominance of different acute phase proteins at different time points based on differential expression analysis. Antimicrobial peptides, notably cathelicidins and peptidoglycan recognition protein, were upregulated at all time points post challenge and peaked at 57 h, which coincided with 10 000-fold decrease in average bacterial counts. The integration of clinical, bacteriological, immunological and quantitative proteomics and other-omic data provides a more detailed systems level view of the host response to mastitis than has been achieved previously.
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DOI：
10.1155/2012/494572
发表时间：
2012
期刊：
International journal of plant genomics
影响因子：
--
作者：
Abdallah C;Dumas-Gaudot E;Renaut J;Sergeant K
通讯作者：
Sergeant K
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
3.3
作者：
Bannerman, D. D.
通讯作者：
Bannerman, D. D.
影响因子：
2.1
作者：
Akerstedt, Maria;Wredle, Ewa;Johansson, Monika
通讯作者：
Johansson, Monika
影响因子：
6.4
作者：
Aebersold, R
通讯作者：
Aebersold, R