Antimicrobial activities of a small molecule compound II-6s against oral streptococci.
Antimicrobial activities of a small molecule compound II-6s against oral streptococci.
复制标题
小分子化合物II-6s对口腔链球菌的抗菌活性
DOI：
10.1080/20002297.2021.1909917
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发表时间：
2021-03-30
影响因子：
4.5
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Zhang J;Kuang X;Zhou Y;Yang R;Zhou X;Peng X;Luo Y;Xu X
背景：目前像氯己定（CHX）这类抗菌剂的副作用以及耐药性的出现，使得开发控制龋齿的替代药物成为必要。
目的：本研究开发了一种新型小分子，即II - 6s，并研究了其对与龋齿相关的常见口腔链球菌的抗菌活性。
方法：通过微量稀释法、时间 - 杀菌试验和扫描电子显微镜评估链球菌对II - 6s的敏感性。通过共聚焦激光扫描显微镜、荧光原位杂交和定量PCR分析经II - 6s处理的变形链球菌/戈登链球菌/血链球菌3种细菌生物膜的胞外多糖、死活细菌以及细菌组成。通过横向显微放射照相术和CCK - 8检测分别评估II - 6s的抗脱矿作用和细胞毒性。对变形链球菌重复暴露于II - 6s，以评估II - 6s是否会诱导耐药性。
结果：II - 6s对变形链球菌、戈登链球菌和血链球菌表现出与CHX相似的抗菌活性，并显著抑制3种链球菌生物膜的胞外多糖产生、活菌以及脱矿能力。此外，与CHX相比，II - 6s的细胞毒性降低，并且在15次传代后未诱导变形链球菌产生耐药性。
结论：II - 6s可能是成功的龋齿管理计划中一个有前景的部分。
Background: The side effects of present antimicrobials like chlorhexidine (CHX) and the emergence of drug resistance necessitate the development of alternative agents to control dental caries. Aim: This study developed a novel small molecule, namely II-6s, and investigated its antimicrobial activities against common oral streptococci associated with dental caries. Methods: The susceptibility of streptococci to II-6s was evaluated by the microdilution method, time-kill assay and scanning electron microscopy. The exopolysaccharides, dead/live bacteria and bacterial composition of the II-6s-treated Streptococcus mutans/Streptococcus gordonii/Streptococcus sanguinis 3-species biofilms were analyzed by confocal laser scanning microscopy, fluorescent in situ hybridization and quantitative PCR. The anti-demineralization effect and cytotoxicity of II-6s were evaluated by transverse microradiography and CCK-8 assay, respectively. Repeated exposure of S. mutans to II-6s was performed to assess if II-6s could induce drug resistance. Results: II-6s exhibited antimicrobial activity similar to CHX against S. mutans, S. gordonii and S. sanguinis and significantly inhibited exopolysaccharides production, live bacteria and the demineralizing capability of the 3-species streptococcal biofilms. Besides, II-6s showed reduced cytotoxicity relative to CHX and did not induce drug resistance in S. mutans after 15 passages. Conclusion: - II-6s may serve as a promising part of a successful caries management plan.
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影响因子：
3.7
作者：
Ikai H;Odashima Y;Kanno T;Nakamura K;Shirato M;Sasaki K;Niwano Y
通讯作者：
Niwano Y
影响因子：
3.4
作者：
Cieplik F;Kara E;Muehler D;Enax J;Hiller KA;Maisch T;Buchalla W
通讯作者：
Buchalla W
影响因子：
4.9
作者：
Koo, H;Rosalen, PL;Bowen, WH
通讯作者：
Bowen, WH
影响因子：
6.7
作者：
Charles, CH;Mostler, KM;Mankodi, SM
通讯作者：
Mankodi, SM
DOI：
10.1038/nrmicro.2017.99
发表时间：
2017-12
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Koo H;Allan RN;Howlin RP;Stoodley P;Hall-Stoodley L
通讯作者：
Hall-Stoodley L