Antimicrobial efficacy of alternative compounds for use in oral care toward biofilms from caries-associated bacteria in vitro.
Antimicrobial efficacy of alternative compounds for use in oral care toward biofilms from caries-associated bacteria in vitro.
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DOI：
10.1002/mbo3.695
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发表时间：
2019-04
期刊：
影响因子：
3.4
通讯作者：
Buchalla W
中科院分区：
文献类型：
--
作者：
Cieplik F;Kara E;Muehler D;Enax J;Hiller KA;Maisch T;Buchalla W
对于龋活跃患者，除了机械性去除生物膜外，抗菌措施可能也有用。本研究的目的是调查用于口腔护理的两类主要替代化合物（即防腐剂和天然化合物）对与龋齿相关细菌形成的生物膜的抗菌功效，并与口腔护理的金标准葡萄糖酸氯己定（CHX）、氯化十六烷基吡啶（CPC）和锌进行比较。在初始的变形链球菌生物膜中对化合物进行筛选。然后，在成熟的变形链球菌以及包含内氏放线菌、溶齿放线菌和变形链球菌的多菌种生物膜中进一步研究最有效的化合物。在此，对不同的处理时间和浓度进行了评估。通过扫描电子显微镜对生物膜进行可视化观察，并通过流式细胞术以及用SYBR Green和碘化丙啶染色来评估细菌细胞膜损伤。与CHX和CPC相比，柑橘提取物是唯一一种在初始变形链球菌生物膜中表现出相似抗菌功效（>5 log10）的化合物，但其在成熟变形链球菌和多菌种生物膜中的效果明显较差。流式细胞术数据表明，柑橘提取物的抗菌作用机制可能基于对细菌细胞膜的损伤，这与CHX和CPC类似。在本研究中所调查的所有替代化合物中，柑橘提取物对来自龋齿相关细菌的体外生物膜表现出最高的抗菌功效，但仍然不如口腔护理的金标准抗菌剂CHX和CPC有效。然而，柑橘提取物可能是一种对龋活跃患者的口腔护理有价值的抗菌化合物。
For caries‐active patients, antimicrobial measures may be useful in addition to mechanical biofilm removal. The aim of this study was to investigate the antimicrobial efficacy of alternative compounds for use in oral care from two main categories (i.e., preservatives and natural compounds) toward biofilms from caries‐associated bacteria as compared to oral care gold‐standards chlorhexidine digluconate (CHX), cetylpyridinium chloride (CPC), and zinc. Compounds were screened in initial Streptococcus mutans biofilms. Then, the most effective compounds were further investigated in mature S. mutans and polymicrobial biofilms comprising Actinomyces naeslundii, Actinomyces odontolyticus, and S. mutans. Here, distinct treatment periods and concentrations were evaluated. Biofilms were visualized by scanning electron microscopy and bacterial membrane damage was evaluated by means of flow cytometry and staining with SYBR Green and propidium iodide. Citrus extract was the only compound exhibiting similar antimicrobial efficacy in initial S. mutans biofilms (>5 log10) as compared to CHX and CPC, but its effect was clearly inferior in mature S. mutans and polymicrobial biofilms. Flow cytometric data suggested that the mechanism of antimicrobial action of citrus extract may be based on damage of bacterial membranes similar to CHX and CPC. From all alternative compounds investigated in this study, citrus extract exhibited the highest antimicrobial efficacy toward in vitro biofilms from caries‐associated bacteria, but still was less effective than oral care gold‐standard antiseptics CHX and CPC. Nevertheless, citrus extract may be a valuable antimicrobial compound for use in oral care for caries‐active patients.
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DOI：
10.2174/1874285801307010130
发表时间：
2013-01-01
期刊：
The open microbiology journal
影响因子：
--
作者：
Cormier, Jiemin;Scott, Ronson;Janes, Marlene
通讯作者：
Janes, Marlene
影响因子：
5.2
作者：
Karygianni, Lamprini;Al-Ahmad, Ali;Skaltsounis, Alexios L.
通讯作者：
Skaltsounis, Alexios L.
影响因子：
7.6
作者：
Guggenheim, B;Giertsen, E;Shapiro, S
通讯作者：
Shapiro, S
影响因子：
6.7
作者：
Marsh, P. D.;Zaura, Egija
通讯作者：
Zaura, Egija
影响因子：
5.7
作者：
Fernandez y Mostajo, Mercedes;van der Reijden, Wil A.;Zaura, Egija
通讯作者：
Zaura, Egija