Evaluation of high mobility group box 1 protein as a presurgical diagnostic marker reflecting the severity of acute appendicitis.
Evaluation of high mobility group box 1 protein as a presurgical diagnostic marker reflecting the severity of acute appendicitis.
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高迁移率族蛋白1蛋白作为反映急性阑尾炎严重程度的术前诊断标志物的评价
DOI：
10.1186/1757-7241-20-61
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发表时间：
2012-09-04
期刊：
影响因子：
--
通讯作者：
Gong J
中科院分区：
文献类型：
--
作者：
Wu C;Sun H;Wang H;Chi J;Liu Q;Guo H;Gong J
目的探讨高迁移率族蛋白B1（HMGB 1）在不同病理严重程度急性阑尾炎（AA）诊断中的价值。每个病例组包括50例性别和年龄相匹配的病例，并与50例健康志愿者进行比较。采用实时荧光定量PCR和酶联免疫吸附试验（ELISA）检测血清HMGB 1 mRNA和蛋白表达水平。结果与健康志愿者相比，第1、2、3组HMGB 1 mRNA相对水平分别升高3.05 ± 0.51、8.33 ± 0.75和13.74 ± 1.09倍，且有加重的趋势; 3组患者血清HMGB 1蛋白水平分别为10.97 ± 1.64、14.42 ± 1.56和18.08 ± 2.41 ng/ml，均显著高于健康志愿者的5.47 ± 0.73 ng/ml。hs-CRP水平分别为12.85 ± 3.41、21.04 ± 1.98和31.07 ± 5.46ng/ml，对照组为2.06 ± 0.77ng/ml。HMGB 1和hs-CRP水平与AA病情严重程度呈正相关。结论血清HMGB 1可作为AA诊断的一项有价值的指标。HMGB 1的mRNA和蛋白表达与hs-CRP水平呈正相关，在一定程度上反映了AA的严重程度。
ObjectivesTo validate the role of high mobility group box-1(HMGB1) in diagnosis of acute appendicitis (AA) with different pathological severity.MethodsAccording to the pathologically diagnosis, 150 patients underwent appendectomies between Jan. 2007 and Dec, 2010 were divided into acute simple, acute suppurative and acute gangrenous appendicitis as group 1, 2 and 3, respectively. Each patient group contains 50 sex and age matched cases to make comparison with 50 healthy volunteers. The mRNA and protein expression levels of serum HMGB1 were determined by real-time quantitative PCR and enzyme linked immunosorbent assay (ELISA). Serum High-sensitivity C-reactive protein (hs-CRP) levels were determined by rate nephelometric immunoassay.ResultsIn comparison with health volunteers, relative HMGB1 mRNA levels in group 1, 2 and 3 were significantly increased 3.05 ± 0.51,8.33 ± 0.75 and 13.74 ± 1.09 folds, reflecting a tendency of augmented severity. In accordance, serum protein levels of HMGB1 were 10.97 ± 1.64, 14.42 ± 1.56 and 18.08 ± 2.41 ng/ml in 3 patient groups, which are significantly higher than that of healthy volunteers’ 5.47 ± 0.73 ng/ml. hs-CRP levels were 12.85 ± 3.41, 21.04 ± 1.98 and 31.07 ± 5.46 ng/ml in 3 patients groups compared with 2.06 ± 0.77 ng/ml in controls. The concentrations of HMGB1 and hs-CRP were both positively correlated with disease severity.ConclusionSerum HMGB1 constitutes as a valuable marker in diagnosis of AA. Positively correlated with hs-CRP level, mRNA and protein expression of HMGB1 to a certain extent reflected the severity of AA.
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DOI：
10.1186/1757-7241-19-27
发表时间：
2011-04-20
期刊：
Scandinavian journal of trauma, resuscitation and emergency medicine
影响因子：
--
作者：
Albayrak Y;Albayrak A;Celik M;Gelincik I;Demiryılmaz I;Yildirim R;Ozogul B
通讯作者：
Ozogul B
影响因子：
4.4
作者：
Li, Wei;Li, Jianhua;Wang, Haichao
通讯作者：
Wang, Haichao
DOI：
10.1164/ajrccm.164.10.2106117
发表时间：
2001-11-15
影响因子：
24.7
作者：
Wang, HH;Yang, H;Tracey, KJ
通讯作者：
Tracey, KJ
影响因子：
1.6
作者：
Sand, M.;Trullen, X. V.;Mann, B.
通讯作者：
Mann, B.
DOI：
10.1186/cc8141
发表时间：
2009
期刊：
Critical care (London, England)
影响因子：
--
作者：
Abraham E
通讯作者：
Abraham E