Beyond the average: An updated framework for understanding the relationship between cell growth, DNA replication, and division in a bacterial system.
Beyond the average: An updated framework for understanding the relationship between cell growth, DNA replication, and division in a bacterial system.
复制标题
DOI：
10.1371/journal.pgen.1010505
复制
发表时间：
2023-01
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
我们对细菌细胞周期的理解在很大程度上是基于群体的实验，这些实验侧重于理想化的平均细胞的行为。最著名的是，库珀和赫尔姆施泰特的贡献有助于了解在快速生长的细菌中观察到的重叠复制周期的现象。尽管这些方法具有不可否认的价值，但它们对理想化平均细胞行为的必要依赖掩盖了单细胞生长和生理所固有的随机性，并限制了它们的机械价值。为了弥补这一差距，我们提出了一个更新的和不可知性的框架，根据现有的单细胞数据，定量地解释了单细胞动力学中的随机变化以及培养基组分对细胞生长和细胞周期进程的影响。在这个框架中，对介质组成敏感的随机计时器影响细胞周期事件之间的关系，解释了在生长缓慢和快速生长的细胞中观察到的细胞周期事件之间的关系差异。我们总结了一个路线图的潜在应用该框架的长期悬而未决的问题在细菌细胞周期领域。
Our understanding of the bacterial cell cycle is framed largely by population-based experiments that focus on the behavior of idealized average cells. Most famously, the contributions of Cooper and Helmstetter help to contextualize the phenomenon of overlapping replication cycles observed in rapidly growing bacteria. Despite the undeniable value of these approaches, their necessary reliance on the behavior of idealized average cells masks the stochasticity inherent in single-cell growth and physiology and limits their mechanistic value. To bridge this gap, we propose an updated and agnostic framework, informed by extant single-cell data, that quantitatively accounts for stochastic variations in single-cell dynamics and the impact of medium composition on cell growth and cell cycle progression. In this framework, stochastic timers sensitive to medium composition impact the relationship between cell cycle events, accounting for observed differences in the relationship between cell cycle events in slow- and fast-growing cells. We conclude with a roadmap for potential application of this framework to longstanding open questions in the bacterial cell cycle field.
登录
查看更多内容
DOI：
10.1016/j.cub.2017.09.046
发表时间：
2017-11-06
期刊：
Current biology : CB
影响因子：
--
作者：
Logsdon MM;Ho PY;Papavinasasundaram K;Richardson K;Cokol M;Sassetti CM;Amir A;Aldridge BB
通讯作者：
Aldridge BB
细胞大小和细菌中DNA复制的启动。
影响因子：
4.5
作者：
Hill NS;Kadoya R;Chattoraj DK;Levin PA
通讯作者：
Levin PA
影响因子：
3.5
作者：
Jameson KH;Wilkinson AJ
通讯作者：
Wilkinson AJ
DOI：
10.1088/1361-6633/aaa628
发表时间：
2018-05
期刊：
Reports on progress in physics. Physical Society (Great Britain)
影响因子：
--
作者：
Jun S;Si F;Pugatch R;Scott M
通讯作者：
Scott M
影响因子：
5.6
作者：
CAIRNS, J;SINSHEIMER, RL
通讯作者：
SINSHEIMER, RL