Smartphone compatible nitric oxide releasing insert to prevent catheter-associated infections.
Smartphone compatible nitric oxide releasing insert to prevent catheter-associated infections.
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DOI：
10.1016/j.jconrel.2022.06.043
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发表时间：
2022-09
影响因子：
10.8
通讯作者：
Brisbois, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Chug, Manjyot Kaur;Brisbois, Elizabeth J.
在免疫功能低下的患者中，很大一部分医院感染与被认为危及生命的医疗器械有关。医疗器械相关感染是细菌定植和设备表面生物膜形成的结果，仅在美国每年就有100万人受到影响。在过去的几年中，基于光的抗菌治疗在解决微生物定植方面取得了实质性进展。利用一氧化氮（NO）的光和抗菌特性，首次采用一种强大的、生物相容性的、抗感染的方法设计了一种既能防止细菌粘附又能就地消毒留置导管的通用一次性导管消毒插入物（DCDI）。DCDI采用光致NO供体分子，聚合物管安装在与LED光源相连的侧发光光纤上。使用智能手机应用程序，通过白光激活DCDI释放NO，导致NO的生理水平可调长达24小时。当受到金黄色葡萄球菌和大肠杆菌的微生物攻击时，与仅暴露4小时的对照组相比，释放NO的DCDI在统计上减少了99%的微生物附着。DCDI还根除了CVC导管模型上约97%的预定植细菌，表明能够消灭已建立的导管感染。这种智能的、可移动操作的新型通用抗菌装置既可用于预防导管感染，也可插入感染导管内以根除细菌，无需复杂的手术干预。通过照明光纤产生的治疗水平的NO可以成为导管相关血流感染的下一代生物相容性解决方案。
A large fraction of nosocomial infections is associated with medical devices that are deemed life-threatening in immunocompromised patients. Medical device-related infections are a result of bacterial colonization and biofilm formation on the device surface that affects >1 million people annually in the US alone. Over the past few years, light-based antimicrobial therapy has made substantial advances in tackling microbial colonization. Taking the advantage of light and antibacterial properties of nitric oxide (NO), for the first time, a robust, biocompatible, anti-infective approach to design a universal disposable catheter disinfection insert (DCDI) that can both prevent bacterial adhesion and disinfect indwelling catheters in situ is reported. The DCDI is engineered using a photo-initiated NO donor molecule, incorporated in polymer tubing that is mounted on a side glow fiber optic connected to an LED light source. Using a smartphone application, the NO release from DCDI is photo-activated via white light resulting in tunable physiological levels of NO for up to 24 h. When challenged with microorganisms S. aureus and E. coli, the NO-releasing DCDI statistically reduced microbial attachment by >99% versus the controls with just 4 h of exposure. The DCDI also eradicated ~97% of pre-colonized bacteria on the CVC catheter model demonstrating the ability to exterminate an established catheter infection. The smart, mobile-operated novel universal antibacterial device can be used to both prevent catheter infections or can be inserted within an infected catheter to eradicate the bacteria without complex surgical interventions. The therapeutic levels of NO generated via illuminating fiber optics can be the next-generation biocompatible solution for catheter-related bloodstream infections.
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影响因子：
9.7
作者：
Brisbois EJ;Major TC;Goudie MJ;Bartlett RH;Meyerhoff ME;Handa H
通讯作者：
Handa H
影响因子：
11.8
作者：
Darouiche, RO
通讯作者：
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2.5
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影响因子：
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作者：
Chug, Manjyot Kaur;Feit, Corbin;Brisbois, Elizabeth J.
通讯作者：
Brisbois, Elizabeth J.
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3.2
作者：
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