Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment.
Application of M1 macrophage as a live vector in delivering nanoparticles for in vivo photothermal treatment.
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DOI：
10.1016/j.jare.2021.01.010
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发表时间：
2021-07
影响因子：
10.7
通讯作者：
Baek SK
中科院分区：
文献类型：
--
作者：
Im NR;Yang TD;Park K;Lee JH;Lee J;Hyuck Kim Y;Lee JS;Kim B;Jung KY;Choi Y;Baek SK
为了提高光热治疗（PTT）效率，使用细胞载体进行纳米颗粒（NPs）递送的递送方法近年来引起了人们的关注和广泛研究。在本研究中，我们证明了 M1 激活的巨噬细胞作为活体载体来递送 NP 的可行性，并研究了脂多糖刺激的负载 NP 的 M1 对体内 PTT 效率的影响。 M1 被用作递送 NP 的活载体，并进一步研究负载 M1 的 NP 对 PTT 效率的影响。未活化的巨噬细胞 (MФ) 被脂多糖 (LPS) 刺激进入 M1，并评估肿瘤细胞对 NP 的吞噬能力。我们发现，与未活化的巨噬细胞 MФ 相比，M1 每细胞体积对 NP 的摄取能力高 20 倍，对肿瘤部位的浸润性高 2.9 倍。我们在瘤周注射 M1 细胞，观察到这些细胞在 12 小时内渗透到肿瘤块中。然后，我们使用近红外激光以 1 W/cm2 照射 1 分钟进行 PTT。结果，我们证实，使用 M1 作为活性活载体可在 1 天内使肿瘤大小更快减小，这表明负载 NPs 的 M1 的 PTT 疗效高于负载 NPs 的 MФ。我们的研究证明了 M1 作为活载体在增强 PTT 在癌症治疗中的可行性方面的潜在作用。
To enhance photothermal treatment (PTT) efficiency, a delivery method that uses cell vector for nanoparticles (NPs) delivery has drawn attention and studied widely in recent years. In this study, we demonstrated the feasibility of M1 activated macrophage as a live vector for delivering NPs and investigated the effect of NPs loaded M1 stimulated by Lipopolysaccharide on PTT efficiency in vivo. M1 was used as a live vector for delivering NPs and further to investigate the effect of NPs loaded M1 on PTT efficiency. Non-activated macrophage (MФ) was stimulated by lipopolysaccharide (LPS) into M1 and assessed for tumor cell phagocytic capacity towards NPs We found M1 exhibited a 20-fold higher uptake capacity of NPs per cell volume and 2.9-fold more active infiltration into the tumor site, compared with non-activated macrophage MФ. We injected M1 cells peritumorally and observed that these cells penetrated into the tumor mass within 12 h. Then, we conducted PTT using irradiation of a near-infrared laser for 1 min at 1 W/cm2. As a result, we confirmed that using M1 as an active live vector led to a more rapid reduction in tumor size within 1 day indicating that the efficacy of PTT with NPs-loaded M1 is higher than that with NPs-loaded MФ. Our study demonstrated the potential role of M1 as a live vector for enhancing the feasibility of PTT in cancer treatment.
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影响因子：
9.9
作者：
Barrera, Carola;Herrera, Adriana P.;Rinaldi, Carlos
通讯作者：
Rinaldi, Carlos
DOI：
10.1016/s0360-3016(00)00462-4
发表时间：
2000-05-01
影响因子：
7
作者：
Cheng, JCH;Chuang, VP;Horng, CF
通讯作者：
Horng, CF
DOI：
10.1073/pnas.2232479100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Hirsch, LR;Stafford, RJ;West, JL
通讯作者：
West, JL
影响因子：
3.7
作者：
Heinrich F;Lehmbecker A;Raddatz BB;Kegler K;Tipold A;Stein VM;Kalkuhl A;Deschl U;Baumgärtner W;Ulrich R;Spitzbarth I
通讯作者：
Spitzbarth I
影响因子：
1.3
作者：
Mills, Charles D.
通讯作者：
Mills, Charles D.