Development of a Multifunctional Bioerodible Nanocomposite Containing Metronidazole and Curcumin to Apply on L-PRF Clot to Promote Tissue Regeneration in Dentistry.
Development of a Multifunctional Bioerodible Nanocomposite Containing Metronidazole and Curcumin to Apply on L-PRF Clot to Promote Tissue Regeneration in Dentistry.
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DOI：
10.3390/biomedicines8100425
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发表时间：
2020-10-16
期刊：
影响因子：
4.7
通讯作者：
De Caro V
中科院分区：
文献类型：
--
作者：
Murgia D;Angellotti G;Conigliaro A;Carfi Pavia F;D'Agostino F;Contardi M;Mauceri R;Alessandro R;Campisi G;De Caro V
拔牙后通常会发生牙槽骨重吸收，尽管通过牙种植体进行可靠的修复需要足够的骨水平。富含白细胞和血小板的纤维蛋白（L-PRF）已被广泛应用于再生过程，并与抗生素和抗氧化剂一起在硬组织和软组织愈合中发挥重要作用。在这项工作中，由装载有甲硝唑（MTR）的透明质酸盐基海绵和含有姜黄素的纳米结构脂质载体（CUR-NLC）组成的纳米复合材料（Sponge-C-MTR）被设计成在提取后插座中包裹在L-PRF™膜中并进行表征。通过均质化然后对脂质混合物进行高频超声处理获得的CUR-NLC显示出负载能力（5%w/w）、药物回收率（95%w/w）、平均粒径为112.0 nm的球形形状和−24 mV的Zeta电位。海绵-C-MTR是通过冷冻干燥工艺将CUR-NLC包埋在亲水性基质中获得的，并评价了理化和细胞相容性特性。此外，评估了CUR和MTR渗透和/或渗透L-PRF™和猪颊组织的能力，突出了系统促进的MTR渗透和CUR积累。结果积极支持当与L-PRF™一起应用时，纳米复合材料在牙齿组织再生中的作用。
Teeth extractions are often followed by alveolar bone reabsorption, although an adequate level of bone is required for reliable rehabilitations by dental implants. Leukocyte and platelet-rich fibrin (L-PRF) has been widely applied in regenerative procedures and with antibiotic and antioxidant agents could play an essential role in hard and soft tissue healing. In this work, a nanocomposite (Sponge-C-MTR) consisting of a hyaluronate-based sponge loaded with metronidazole (MTR) and nanostructured lipid carriers containing curcumin (CUR-NLC) was designed to be wrapped in the L-PRF™ membrane in the post-extraction sockets and characterized. CUR-NLCs, obtained by homogenization followed by high-frequency sonication of the lipid mixture, showed loading capacity (5% w/w), drug recovery (95% w/w), spherical shape with an average particle size of 112.0 nm, and Zeta potential of −24 mV. Sponge-C-MTR was obtained by entrapping CUR-NLC in a hydrophilic matrix by a freeze-drying process, and physico-chemical and cytocompatibility properties were evaluated. Moreover, the aptitude of CUR and MTR to the penetrate and/or permeate both L-PRF™ and porcine buccal tissue was assessed, highlighting MTR penetration and CUR accumulation promoted by the system. The results positively support the action of nanocomposite in dental tissues regeneration when applied together with the L-PRF™.
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影响因子：
5.4
作者：
De Caro, Viviana;Murgia, Denise;Campisi, Giuseppina
通讯作者：
Campisi, Giuseppina
影响因子：
4.3
作者：
Barone, A.;Ricci, M.;Covani, U.
通讯作者：
Covani, U.
DOI：
10.1016/b978-1-4557-2834-3.00017-3
发表时间：
2013-01-01
期刊：
HANDBOOK OF BIOPOLYMERS AND BIODEGRADABLE PLASTICS: PROPERTIES, PROCESSING AND APPLICATIONS
影响因子：
--
作者：
Kramschuster, Adam;Turng, Lih-Sheng
通讯作者：
Turng, Lih-Sheng
影响因子：
--
作者：
Cano-Duran, Jorge A;Pena-Cardelles, Juan-Francisco;Lopez-Quiles, Juan
通讯作者：
Lopez-Quiles, Juan
DOI：
10.1002/psp4.12300
发表时间：
2018-07
期刊：
CPT: pharmacometrics & systems pharmacology
影响因子：
--
作者：
Dallmann A;Solodenko J;Ince I;Eissing T
通讯作者：
Eissing T