Localization and quantitative co-localization of enamelin with amelogenin.
Localization and quantitative co-localization of enamelin with amelogenin.
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DOI：
10.1016/j.jsb.2013.03.014
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发表时间：
2013-08
影响因子：
3
通讯作者：
Moradian-Oldak, Janet
中科院分区：
文献类型：
--
作者：
Gallon, Victoria;Chen, Lisha;Yang, Xiudong;Moradian-Oldak, Janet
关键词：
釉蛋白和成釉原蛋白是牙釉质形成的重要蛋白质。这两种蛋白的协同作用控制着体外晶体的成核和形态。我们利用共聚焦显微镜和两种抗体定量分析了釉素和淀粉原蛋白之间的共定位，一种抗体针对猪32 kDa的釉素区域的一个序列，另一种针对全长重组小鼠淀粉原蛋白。我们利用免疫金标技术进一步研究了猪32kda釉蛋白与重组淀粉原蛋白的相互作用。本研究报告了出生后1、2、3、4、5、6、7和8天小鼠下颌磨牙的定量共定位结果。我们发现，在第1天，磨牙尖坡的细胞外基质分泌出淀粉原蛋白和釉素，这种分泌至少持续到第8天。利用大（45 μm高，33 μm宽）和小（7 μm直径）感兴趣的区域在几种不同的配置下进行定量共定位分析（QCA），以阐明任何模式。第8天样品的共定位模式显示，釉素和成釉原蛋白共定位在成釉细胞的分泌面附近，并以大约1:1的比例分泌。随着牙釉质的成熟，沿成釉细胞的分泌面和整个牙釉质的厚度，共定位的程度降低。针对珐琅质的免疫反应性集中在成釉细胞的分泌面，这支持了该蛋白与成釉原蛋白密切参与珐琅质形成初期矿物诱导的理论。
Enamelin and amelogenin are vital proteins in enamel formation. The cooperative function of these two proteins controls crystal nucleation and morphology in vitro. We quantitatively analyzed the co-localization between enamelin and amelogenin by confocal microscopy and using two antibodies, one raised against a sequence in the porcine 32 kDa enamelin region and the other raised against full-length recombinant mouse amelogenin. We further investigated the interaction of the porcine 32 kDa enamelin and recombinant amelogenin using immuno-gold labeling. This study reports the quantitative co-localization results for postnatal days 1, 2, 3, 4, 5, 6, 7 and 8 mandibular mouse molars. We show that amelogenin and enamelin are secreted into the extracellular matrix on the cuspal slopes of the molars at day 1 and that secretion continues to at least day 8. Quantitative co-localization analysis (QCA) was performed in several different configurations using large (45 μm height, 33 μm width) and small (7 μm diameter) regions of interest to elucidate any patterns. Co-localization patterns in day 8 samples revealed that enamelin and amelogenin co-localize near the secretory face of the ameloblasts and appear to be secreted approximately in a 1:1 ratio. The degree of co-localization decreases as the enamel matures, both along the secretory face of ameloblasts and throughout the entire thickness of the enamel. Immuno-reactivity against enamelin is concentrated along the secretory face of ameloblasts, supporting the theory that this protein together with amelogenin is intimately involved in mineral induction at the beginning of enamel formation.
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影响因子：
7.6
作者：
Bouropoulos, N;Moradian-Oldak, J
通讯作者：
Moradian-Oldak, J
影响因子：
3.8
作者：
Iijima, Mayumi;Fan, Daming;Bromley, Keith M.;Sun, Zhi;Moradian-Oldak, Janet
通讯作者：
Moradian-Oldak, Janet
影响因子：
3
作者：
He, Xiaodong;Li, Wu;Habelitz, Stefan
通讯作者：
Habelitz, Stefan
影响因子：
4.8
作者：
Bromley, Keith M.;Kiss, Andrew S.;Moradian-Oldak, Janet
通讯作者：
Moradian-Oldak, Janet
通过可变温度圆形二色性和等温滴定量热法对二级结构和自组装的分析。
影响因子：
2.9
作者：
Lakshminarayanan, Rajamani;Yoon, Il;Hegde, Balachandra G.;Fan, Daming;Du, Chang;Moradian-Oldak, Janet
通讯作者：
Moradian-Oldak, Janet