Tooth enamel proteins enamelin and amelogenin cooperate to regulate the growth morphology of octacalcium phosphate crystals.
Tooth enamel proteins enamelin and amelogenin cooperate to regulate the growth morphology of octacalcium phosphate crystals.
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DOI：
10.1021/cg100696r
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发表时间：
2010-11
影响因子：
3.8
通讯作者：
Moradian-Oldak, Janet
中科院分区：
文献类型：
--
作者：
Iijima, Mayumi;Fan, Daming;Bromley, Keith M.;Sun, Zhi;Moradian-Oldak, Janet
为了研究釉质蛋白和淀粉原蛋白在分泌后控制釉质晶体生长形态中的协同作用，我们采用阳离子选择膜系统，在含有和不含32kDa淀粉原片段的重组猪淀粉原蛋白（rP148）中培养磷酸八钙（OCP）。搪瓷素对OCP晶体c轴方向生长的抑制作用大于rP148，产生的OCP晶体长宽比最小。当釉素加入到淀粉原蛋白“凝胶样基质”中时，蛋白混合物对OCP在c轴方向上生长的抑制作用减弱，而在b轴方向上的抑制作用增强。结果表明，OCP晶体的长宽比（长宽比）明显提高。在淀粉原中加入釉素增强了淀粉原稳定无定形磷酸钙（ACP）瞬相的潜力。釉素和淀粉原蛋白的比例对ACP的稳定和大长宽比OCP晶体的生长至关重要。釉质蛋白和淀粉原蛋白的协同调控作用可能是通过釉质蛋白和淀粉原蛋白的协同组装实现的。这些结果对了解大纵横比釉质晶体的生长机理具有重要意义。
To examine the hypothetical cooperative role of enamelin and amelogenin in controlling the growth morphology of enamel crystals in the post-secretory stage, we applied a cation selective membrane system for the growth of octacalcium phosphate (OCP) in the truncated recombinant porcine amelogenin (rP148) with and without the 32kDa enamelin fragment. Enamelin alone inhibited the growth in the c-axis direction more than rP148, yielding OCP crystals with the smallest aspect ratio of all conditions tested. When enamelin was added to the amelogenin “gel-like matrix”, the inhibitory action of the protein mixture on the growth of OCP in the c-axis direction was diminished, while that in the b-axis direction was increased. As a result, the length to width ratio (aspect ratio) of OCP crystal was markedly increased. Addition of enamelin to amelogenin enhanced the potential of amelogenin to stabilize the amorphous calcium phosphate (ACP) transient phase. The ratio of enamelin and amelogenin was crucial for stabilization of ACP and the growth of OCP crystals with larger aspect ratio. The cooperative regulatory action of enamelin and amelogenin was attained, presumably, through co-assembling of enamelin and amelogenin. These results have important implications in understanding the growth mechanism of enamel crystals with large aspect ratio.
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影响因子：
7.6
作者：
Bouropoulos, N;Moradian-Oldak, J
通讯作者：
Moradian-Oldak, J
影响因子：
3
作者：
Beniash, Elia;Metzler, Rebecca A.;Lam, Raymond S. K.;Gilbert, P. U. P. A.
通讯作者：
Gilbert, P. U. P. A.
影响因子：
4.1
作者：
Crane, Nicole J.;Popescu, Victoria;Ignelzi, Michael A., Jr.
通讯作者：
Ignelzi, Michael A., Jr.
影响因子：
3
作者：
Fan D;Du C;Sun Z;Lakshminarayanan R;Moradian-Oldak J
通讯作者：
Moradian-Oldak J
影响因子：
62.1
作者：
Gower, Laurie B.
通讯作者：
Gower, Laurie B.