Structural analysis of ATP analogues compatible with kinase-catalyzed labeling.
Structural analysis of ATP analogues compatible with kinase-catalyzed labeling.
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与激酶催化标记兼容的 ATP 类似物的结构分析。
DOI：
10.1021/bc300404s
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发表时间：
2012
影响因子：
4.7
通讯作者：
Pflum,MaryKayH
中科院分区：
文献类型：
--
作者：
Suwal,Sujit;Senevirathne,Chamara;Garre,Satish;Pflum,MaryKayH
Kinase-catalyzed protein phosphorylation is an important biochemical process involved in cellular functions. We recently discovered that kinases promiscuously accept γ-modified ATP analogues as cosubstrates and used several ATP analogues as tools for studying protein phosphorylation. Herein, we explore the structural requirements of γ-modified ATP analogues for kinase compatibility. To understand the influence of linker length and composition, a series of ATP analogues was synthesized, and the efficiency of kinase-catalyzed labeling was determined by quantitative mass spectrometry. This study on factors influencing kinase cosubstrate promiscuity will enable design of ATP analogues for a variety of kinase-catalyzed labeling reactions.
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影响因子：
16.6
作者：
Suwal, Sujit;Pflum, Mary Kay H.
通讯作者：
Pflum, Mary Kay H.
影响因子：
3
作者：
Trott, Oleg;Olson, Arthur J.
通讯作者：
Olson, Arthur J.
DOI：
10.1002/1097-0282(2001)60:1
发表时间：
2001
期刊：
Biopolymers.
影响因子：
--
作者：
Sun,T;Campbell,M;Gordon,W;Arlinghaus,RB
通讯作者：
Arlinghaus,RB
影响因子：
15
作者：
Green, Keith D.;Pflum, Mary Kay H.
通讯作者：
Pflum, Mary Kay H.
影响因子：
4.8
作者：
Garcia, BA;Shabanowitz, J;Hunt, DF
通讯作者：
Hunt, DF