BrCPS1 Function in Leafy Head Formation Was Verified by Two Allelic Mutations in Chinese Cabbage (Brassica rapa L. ssp. pekinensis).
BrCPS1 Function in Leafy Head Formation Was Verified by Two Allelic Mutations in Chinese Cabbage (Brassica rapa L. ssp. pekinensis).
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BrCPS1 在叶头形成中的功能通过大白菜 (Brassica rapa L. ssp. pekinensis) 中的两个等位基因突变得到验证
DOI：
10.3389/fpls.2022.889798
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发表时间：
2022
影响因子：
5.6
通讯作者：
Feng, Hui
中科院分区：
文献类型：
--
作者：
Gao, Yue;Qu, Gaoyang;Huang, Shengnan;Liu, Zhiyong;Fu, Wei;Zhang, Meidi;Feng, Hui
大白菜叶球的形成是一个重要的农业因素，因为它直接影响产量。本研究从结球白菜双单倍体“FT”的甲基磺酸乙酯诱变群体中鉴定出两个等位不结球突变体nhm 4 -1和nhm 4 -2。利用MutMap、竞争等位基因特异性PCR基因分型和图位克隆，我们发现BraA09g001440.3C是突变体的致病基因。BraA09g001440.3C编码参与赤霉素生物合成的对映-柯巴基二磷酸合酶1。BraA09g001440.3C基因第7和第4外显子中的一个非同义SNP分别导致nhm 4 -1和nhm 4 -2突变表型。与野生型“FT”相比，突变体叶片中赤霉素含量显著降低。这两个突变体均表现出在外源GA 3处理后形成叶状头状花序的趋势。这两个不结球突变体和本文的工作表明，赤霉素与大白菜叶球形成有关。
The formation of the leafy heads of Chinese cabbage is an important agricultural factor because it directly affects yield. In this study, we identified two allelic non-heading mutants, nhm4-1 and nhm4-2, from an ethyl methanesulfonate mutagenic population of a heading Chinese cabbage double haploid line “FT.” Using MutMap, Kompetitive Allele-Specific PCR genotyping, and map-based cloning, we found that BraA09g001440.3C was the causal gene for the mutants. BraA09g001440.3C encodes an ent-copalyl diphosphate synthase 1 involved in gibberellin biosynthesis. A single non-synonymous SNP in the seventh and fourth exons of BraA09g001440.3C was responsible for the nhm4-1 and nhm4-2 mutant phenotypes, respectively. Compared with the wild-type “FT,” the gibberellin content in the mutant leaves was significantly reduced. Both mutants showed a tendency to form leafy heads after exogenous GA3 treatment. The two non-heading mutants and the work presented herein demonstrate that gibberellin is related to leafy head formation in Chinese cabbage.
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影响因子：
5.4
作者：
Liu, Chao;Wang, Jilin;Liu, Kede
通讯作者：
Liu, Kede
影响因子：
11.6
作者：
BENSEN, RJ;JOHAL, GS;BRIGGS, SP
通讯作者：
BRIGGS, SP
影响因子：
44.1
作者：
He, YK;Xue, WX;Liu, PL
通讯作者：
Liu, PL
DOI：
10.1093/bioinformatics/btp698
发表时间：
2010-03-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Li H;Durbin R
通讯作者：
Durbin R
影响因子：
14.9
作者：
Wang K;Li M;Hakonarson H
通讯作者：
Hakonarson H