Effective Connectivity Measured Using Optogenetically Evoked Hemodynamic Signals Exhibits Topography Distinct from Resting State Functional Connectivity in the Mouse.
Effective Connectivity Measured Using Optogenetically Evoked Hemodynamic Signals Exhibits Topography Distinct from Resting State Functional Connectivity in the Mouse.
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DOI：
10.1093/cercor/bhx298
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发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Culver JP
中科院分区：
文献类型：
--
作者：
Bauer AQ;Kraft AW;Baxter GA;Wright PW;Reisman MD;Bice AR;Park JJ;Bruchas MR;Snyder AZ;Lee JM;Culver JP
脑连接组学已从轴突投射连接 (APC) 的组织学评估扩展到包括静息态功能连接 (RS-FC)。 RS-FC 分析对于全脑绘图非常有效，但基于 APC 解释 RS-FC（例如半球间 RS-FC）的各个方面的尝试仅取得了部分成功。仅使用血红蛋白进行神经成像缺乏确定细胞群活动如何促进 RS-FC 的特异性。光遗传学定义的连接的广域映射可以提供对大脑结构与功能关系的见解。我们将光遗传学与光学固有信号成像相结合，创建了一种高效的光遗传学有效连接（Opto-EC）映射分析。我们检查了清醒的 Thy1-ChR2 转基因小鼠兴奋性神经元的 EC 模式。这些基于 Thy1 的 EC (Thy1-EC) 模式与皮层上的 RS-FC 进行了评估。与 RS-FC 相比，Thy1-EC 表现出更高的空间特异性、在具有强 RS-FC 的区域中减少的半球间连接性以及明显的连接强度不对称性。将 Thy1-EC 和 RS-FC 模式的形貌与 APC 的图谱进行比较表明，Thy1-EC 比 RS-FC 更类似于 APC。使用血红蛋白进行 Opto-EC 绘图的更通用方法可以在一小时内确定单个动物的 100 个位点，并且适用于其他神经成像方式。 Opto-EC 映射代表了一种用于检查不断发展的连接相关电路可塑性的强大策略。
Brain connectomics has expanded from histological assessment of axonal projection connectivity (APC) to encompass resting state functional connectivity (RS-FC). RS-FC analyses are efficient for whole-brain mapping, but attempts to explain aspects of RS-FC (e.g., interhemispheric RS-FC) based on APC have been only partially successful. Neuroimaging with hemoglobin alone lacks specificity for determining how activity in a population of cells contributes to RS-FC. Wide-field mapping of optogenetically defined connectivity could provide insights into the brain’s structure–function relationship. We combined optogenetics with optical intrinsic signal imaging to create an efficient, optogenetic effective connectivity (Opto-EC) mapping assay. We examined EC patterns of excitatory neurons in awake, Thy1-ChR2 transgenic mice. These Thy1-based EC (Thy1-EC) patterns were evaluated against RS-FC over the cortex. Compared to RS-FC, Thy1-EC exhibited increased spatial specificity, reduced interhemispheric connectivity in regions with strong RS-FC, and appreciable connection strength asymmetry. Comparing the topography of Thy1-EC and RS-FC patterns to maps of APC revealed that Thy1-EC more closely resembled APC than did RS-FC. The more general method of Opto-EC mapping with hemoglobin can be determined for 100 sites in single animals in under an hour, and is amenable to other neuroimaging modalities. Opto-EC mapping represents a powerful strategy for examining evolving connectivity-related circuit plasticity.
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影响因子：
5.3
作者：
Wright PW;Archambault AS;Peek S;Bauer AQ;Culican SM;Ances BM;Culver JP;Wu GF
通讯作者：
Wu GF
DOI：
10.1523/jneurosci.5845-11.2012
发表时间：
2012-03-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Bero AW;Bauer AQ;Stewart FR;White BR;Cirrito JR;Raichle ME;Culver JP;Holtzman DM
通讯作者：
Holtzman DM
影响因子：
5.7
作者：
Culver, JP;Siegel, AM;Boas, DA
通讯作者：
Boas, DA
DOI：
10.1098/rstb.2005.1634
发表时间：
2005-05-29
影响因子：
6.3
作者：
Beckmann, CF;DeLuca, M;Smith, SM
通讯作者：
Smith, SM
影响因子：
25
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
通讯作者：
Tank, David W.