Fluorinated methacrylamide chitosan hydrogel dressings enhance healing in an acute porcine wound model.
Fluorinated methacrylamide chitosan hydrogel dressings enhance healing in an acute porcine wound model.
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DOI：
10.1371/journal.pone.0203371
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Leipzig ND
中科院分区：
文献类型：
--
作者：
Patil PS;Evancho-Chapman MM;Li H;Huang H;George RL;Shriver LP;Leipzig ND
伤口愈合涉及多个相互关联的过程，这些过程是实现成功愈合结果所必需的。吞噬作用、炎症、细胞增殖、血管生成、能量产生以及胶原蛋白合成都直接或间接依赖于氧气。除了营养和合并症等其他关键因素外，氧气的可获得性是愈合成功的关键决定因素。此前，我们已经提出了一种新型的含氧水凝胶材料，它可以制成敷料，用于向伤口持续局部输送氧气。在这项研究中，使用了一种急性猪伤口模型来测试这些含氧的MACF（MACF + O₂）水凝胶敷料与对照组相比的愈合益处，对照组包括商业的Derma - Gel™水凝胶敷料。进行了伤口闭合和组织学分析，以评估再上皮化、胶原蛋白合成、血管生成和角质形成细胞成熟情况。这些检测的结果显示，与Derma - Gel相比，用MACF + O₂水凝胶敷料处理的伤口闭合更快（p < 0.05）。通过液相色谱分离和质谱检测（LC - MS/MS）进行的靶向代谢组学以及一种生化检测确定了第14天和第21天伤口样本中羟脯氨酸的浓度，结果表明与Derma - Gel相比，MACF + O₂水凝胶敷料通过上调胶原蛋白合成途径改善了伤口愈合（p < 0.05）。组织学证据表明，MACF + O₂水凝胶敷料在所有其他治疗方法中对新血管形成和角质形成细胞成熟的改善效果更佳。
Wound healing involves multiple interrelated processes required to lead to successful healing outcomes. Phagocytosis, inflammation, cell proliferation, angiogenesis, energy production, and collagen synthesis are all directly or indirectly dependent on oxygen. Along with other critical factors, such as nutrition and comorbidities, availability of oxygen is a key determinant of healing success. Previously, we have presented a novel oxygenated hydrogel material that can be made into dressings for continuous localized oxygen delivery to wounds. In this study, an acute porcine wound model was used to test the healing benefits of these oxygenated MACF (MACF + O2) hydrogel dressings compared to controls, which included commercial Derma-GelTM hydrogel dressings. Wound closure and histological analyses were performed to assess re-epithelialization, collagen synthesis, angiogenesis, and keratinocyte maturation. Results from these assays revealed that wounds treated with MACF + O2 hydrogel dressings closed faster as compared to Derma-Gel (p<0.05). Targeted metabolomics via liquid chromatography separation and mass spectrometric detection (LC-MS/MS) and a biochemical assay determined the concentration of hydroxyproline in wound samples at days 14 and 21, showing that MACF + O2 hydrogel dressings improved wound healing via an upregulated collagen synthesis pathway as compared to Derma-Gel (p<0.05). Histological evidence showed that MACF + O2 hydrogel dressings improve new blood vessel formation and keratinocyte maturation over all other treatments.
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