Endogenous versus exogenous markers of adult neurogenesis in canaries and other birds: advantages and disadvantages.
Endogenous versus exogenous markers of adult neurogenesis in canaries and other birds: advantages and disadvantages.
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DOI：
10.1002/cne.23661
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发表时间：
2014-12-15
影响因子：
2.5
通讯作者：
Ball, Gregory F.
中科院分区：
文献类型：
--
作者：
Balthazart, Jacques;Ball, Gregory F.
虽然新生神经元的存在最初被提出，但并未被广泛接受，但根据对成年啮齿动物大脑的研究，成年恒温脊椎动物中活跃的神经发生过程的存在首先在鸣禽中得到证实。成人神经发生最初采用氚化胸苷技术进行研究，后来被注射和检测 DNA 复制标记 5-溴-2'-脱氧尿苷 (BrdU) 技术所取代。最近，各种内源性标记物被用来识别年轻神经元或循环神经元祖细胞。我们在这里回顾了这些不同方法在鸟类中各自的优点和缺陷，特别是微管相关蛋白双皮质素（DCX），它已被广泛用于识别成年大脑中新生的神经元。当然，所有这些技术都有局限性。外源标记物仅在短时间内标记复制其 DNA 的细胞，并且很难选择注射剂量来彻底标记所有这些细胞而不引起 DNA 损伤，而 DNA 损伤也会在修复过程中导致某种形式的标记。另一方面，由于与抗体特异性相关的问题（这些问题可以但并不总是得到解决），内源标记的特异性很难确定，更重要的是，因为很难（如果不是不可能）证明给定的标记能够详尽且特异性地标记给定的细胞群。尽管存在这些潜在的局限性，但这些内源性标记物和 DCX 染色尤其清楚地代表了详细研究神经发生的有用方法，尤其是与 BrdU 等其他技术相结合时。
Although the existence of newborn neurons had originally been suggested, but not broadly accepted, based on studies in adult rodent brains, the presence of an active neurogenesis process in adult homoeothermic vertebrates was first firmly established in songbirds. Adult neurogenesis was initially studied with the tritiated thymidine technique, later replaced by the injection and detection of the marker of DNA replication 5-bromo-2′-deoxyuridine (BrdU). More recently, various endogenous markers were used to identify young neurons or cycling neuronal progenitors. We review here the respective advantages and pitfalls of these different approaches in birds, with specific reference to the microtubule-associated protein, doublecortin (DCX), that has been extensively used to identify young newly born neurons in adult brains. All these techniques of course have limitations. Exogenous markers label cells replicating their DNA only during a brief period and it is difficult to select injection doses that would exhaustively label all these cells without inducing DNA damage that will also result in some form of labeling during repair. On the other hand, specificity of endogenous markers is difficult to establish due to problems related to the specificity of antibodies (these problems can be, but are not always, addressed) and more importantly because it is difficult, if not impossible, to prove that a given marker exhaustively and specifically labels a given cell population. Despite these potential limitations, these endogenous markers and DCX staining in particular clearly represent a useful approach to the detailed study of neurogenesis especially when combined with other techniques such as BrdU.
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影响因子：
56.9
作者：
ALVAREZBUYLLA, A;KIRN, JR;NOTTEBOHM, F
通讯作者：
NOTTEBOHM, F
影响因子：
3.3
作者：
Bauer, S;Hay, M;Moyse, E
通讯作者：
Moyse, E
影响因子：
2.5
作者：
Brown, JP;Couillard-Després, S;Kuhn, HG
通讯作者：
Kuhn, HG
影响因子：
64.8
作者：
BRAVO, R;FRANK, R;MACDONALDBRAVO, H
通讯作者：
MACDONALDBRAVO, H
影响因子：
2.5
作者：
Charvet, Christine J.;Striedter, Georg F.
通讯作者：
Striedter, Georg F.