Arginine deiminase has multiple regulatory roles in the biology of Giardia lamblia.
Arginine deiminase has multiple regulatory roles in the biology of Giardia lamblia.
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DOI：
10.1242/jcs.026963
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Nash, Theodore Elliott
中科院分区：
文献类型：
--
作者：
Carolina Touz, Maria;Silvana Ropolo, Andrea;Romina Rivero, Maria;Veronica Vranych, Cecilia;Conrad, John Thomas;Svard, Staffan Gunnar;Nash, Theodore Elliott
原生动物寄生虫兰氏贾第虫在厌氧条件下利用精氨酸脱亚胺酶（gADI）从游离的l -精氨酸中产生能量。在这项工作中，我们证明了它除了作为一种已知的代谢酶之外，它还可以作为一种肽基精氨酸脱亚胺酶，将蛋白质结合的精氨酸转化为瓜氨酸。gADI特异性结合变异特异性表面蛋白（VSPs）保守的CRGKA尾部的精氨酸并使其瓜氨酸化，影响抗原转换和抗体介导的细胞死亡。在环化过程中，gADI从细胞质转移到细胞核，并在环化特异性基因的表达中发挥调节作用。gADI也被代谢，这可能会调节其活性。我们的研究结果揭示了gADI发挥的双重作用，并定义了贾第虫用于生存的新调控途径。
The protozoan parasite Giardia lamblia utilizes arginine deiminase (gADI) to produce energy from free L-arginine under anaerobic conditions. In this work, we demonstrate that in addition to its known role as a metabolic enzyme, it also functions as a pepidtyl-arginine deiminase converting protein-bound arginine into citrulline. gADI specifically binds to and citrullinates the arginine in the conserved CRGKA tail of variant-specific surface proteins (VSPs) affecting both antigenic switching and antibody mediated cell death. During encystation gADI translocates from the cytoplasm to the nucleus and appear to play a regulatory role in the expression of encystation specific genes. gADI is also sumoylated, which may modulate its activity. Our findings reveal a dual role played by gADI and define novel regulatory pathways used by Giardia for survival.
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影响因子：
3.1
作者：
MOWATT, MR;NGUYEN, BYT;NASH, TE
通讯作者：
NASH, TE
影响因子：
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NASH, TE;CONRAD, JT;MERRITT, JW
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MERRITT, JW
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SCHOFIELD, PJ;EDWARDS, MR;WILSON, JR
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WILSON, JR
影响因子：
1.5
作者：
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作者：
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通讯作者：
Kohler, P