Targeted gene knockouts reveal overlapping functions of the five Physcomitrella patens FtsZ isoforms in chloroplast division, chloroplast shaping, cell patterning, plant development, and gravity sensing.
Targeted gene knockouts reveal overlapping functions of the five Physcomitrella patens FtsZ isoforms in chloroplast division, chloroplast shaping, cell patterning, plant development, and gravity sensing.
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DOI：
10.1093/mp/ssp076
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发表时间：
2009-11
期刊：
影响因子：
27.5
通讯作者：
Reski R
中科院分区：
文献类型：
--
作者：
Martin A;Lang D;Hanke ST;Mueller SJ;Sarnighausen E;Vervliet-Scheebaum M;Reski R
叶绿体和细菌细胞通过二元裂变进行分裂。这种收缩分裂的关键蛋白是 FtsZ，一种类似于真核微管蛋白的自组装 GTP 酶。在原核生物中，FtsZ 几乎总是由单个基因编码，而植物则具有多个核编码的 FtsZ 同源物。在种子植物中，这些蛋白质分为两个家族，并且全部被专门输入到质体中。相比之下，基础陆地植物小立碗藓（一种苔藓）编码第三个 FtsZ 家族，其中有一个成员。该蛋白质双重靶向质体和细胞质。在这里，我们报告了 P. patens 中所有 ftsZ 基因的靶向基因破坏。随后对单敲除和双敲除突变体的分析揭示了不同 FtsZ 亚型之间复杂的相互作用，不仅在质体分裂中，而且在叶绿体成形、细胞模式、植物发育和重力感应中。这些结果支持了质体骨架的概念及其与细胞骨架的功能整合，至少在苔藓 P. patens 中是这样。
Chloroplasts and bacterial cells divide by binary fission. The key protein in this constriction division is FtsZ, a self-assembling GTPase similar to eukaryotic tubulin. In prokaryotes, FtsZ is almost always encoded by a single gene, whereas plants harbor several nuclear-encoded FtsZ homologs. In seed plants, these proteins group in two families and all are exclusively imported into plastids. In contrast, the basal land plant Physcomitrella patens, a moss, encodes a third FtsZ family with one member. This protein is dually targeted to the plastids and to the cytosol. Here, we report on the targeted gene disruption of all ftsZ genes in P. patens. Subsequent analysis of single and double knockout mutants revealed a complex interaction of the different FtsZ isoforms not only in plastid division, but also in chloroplast shaping, cell patterning, plant development, and gravity sensing. These results support the concept of a plastoskeleton and its functional integration into the cytoskeleton, at least in the moss P. patens.
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