Overexpression of the cytoplasmic retention signal region of cyclin B2, but not of cyclin B1, inhibits bipolar spindle formation in Xenopus oocytes
Overexpression of the cytoplasmic retention signal region of cyclin B2, but not of cyclin B1, inhibits bipolar spindle formation in Xenopus oocytes
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细胞周期蛋白 B2 的细胞质保留信号区域的过表达（而非细胞周期蛋白 B1 的细胞质保留信号区域）抑制非洲爪蟾卵母细胞中双极纺锤体的形成
DOI：
10.1111/j.1768-322x.1998.tb01060.x
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发表时间：
1998
影响因子：
2.7
通讯作者：
N. Sagata
中科院分区：
文献类型：
--
作者：
Satoshi Yoshitome;N. Furuno;N. Sagata
细胞周期蛋白B是成熟/M期促进因子（MPF）的调节亚基，在许多脊椎动物物种中具有几种亚型。然而，目前尚不清楚不同的B-型细胞周期蛋白在脊椎动物细胞中是否有任何不同的功能，尽管它们的亚细胞定位似乎彼此差异很大。为了研究两种主要的B-型细胞周期蛋白B1和B2在M期纺锤体形成中的作用，我们在非洲爪蟾卵母细胞中过表达了它们的N-末端;细胞周期蛋白B1和B2的N-末端含有细胞质滞留信号（CRS），因此它们的过表达有望分别竞争性抑制内源性细胞周期蛋白B1和B2的亚细胞定位。在进入减数分裂I时，过表达细胞周期蛋白B1 N末端的卵母细胞形成了一个明显正常的双极纺锤体，但那些过表达细胞周期蛋白B1 N末端的卵母细胞形成了一个单极（或单星）纺锤体。这种双极纺锤体形成的缺陷仅在细胞周期蛋白B2 N末端包含其自身的CRS序列时观察到，并且能够通过全长细胞周期蛋白B2的过表达来挽救。这些结果表明，第一次，正确的亚细胞定位的细胞周期蛋白B2，但不是细胞周期蛋白B1，是必不可少的（启动）双极纺锤体形成爪蟾卵母细胞。
Cyclin B, a regulatory subunit of maturation/M‐phase promoting factor (MPF), has several subtypes in many vertebrate species. However, it is not known whether the different B‐type cyclins have any different functions in vertebrate cells, although their subcellular localizations seem to differ largely from each other. To examine the roles of two major B‐type cyclins, B1 and B2, in spindle formation in M phase, we overexpressed their N‐termini in Xenopus oocytes; the N‐termini of cyclins B1 and B2 contained a cytoplasmic retention signal (CRS), and hence their overexpressions were expected to competitively inhibit the subcellular localizations of the endogenous cyclins B1 and B2, respectively. Upon entry into meiosis I, oocytes overexpressing the cyclin B1 N‐terminus formed an apparently normal bipolar spindle, but those oocytes overexpressing the cyclin B1 N‐terminus formed a monopolar (or monoastral) spindle. This defect in bipolar spindle formation was observed only when the cyclin B2 N‐terminus contained its own CRS sequence, and was able to be rescued by overexpression of full‐length cyclin B2. These results suggest, for the first time, that the correct subcellular localization of cyclin B2, but not of cyclin B1, is essential for (the initiation of) bipolar spindle formation in Xenopus oocytes.
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影响因子：
7.5
作者：
AULT, JG;RIEDER, CL
通讯作者：
RIEDER, CL
DOI：
10.1073/pnas.85.9.3009
发表时间：
1988-05-01
影响因子：
11.1
作者：
LOHKA, MJ;HAYES, MK;MALLER, JL
通讯作者：
MALLER, JL
影响因子：
56.9
作者：
HARTWELL, LH;WEINERT, TA
通讯作者：
WEINERT, TA
影响因子：
11.4
作者：
PINES, J;HUNTER, T
通讯作者：
HUNTER, T