Abnormal alterations of miR-1 and miR-214 are associated with clinicopathological features and prognosis of patients with PDAC.
Abnormal alterations of miR-1 and miR-214 are associated with clinicopathological features and prognosis of patients with PDAC.
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DOI：
10.3892/ol.2017.6819
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发表时间：
2017-10
期刊：
影响因子：
2.9
通讯作者：
Li JB
中科院分区：
文献类型：
--
作者：
Cheng Q;Han LH;Zhao HJ;Li H;Li JB
胰腺导管腺癌（PDAC）是一种预后不良的恶性肿瘤。众所周知，PDAC在早期难以诊断，无复发预后差，但也缺乏有效的治疗方法，对其生物学特性的了解有限。因此，迫切需要进一步了解与PDAC相关的细胞或分子特性，并探索诊断和治疗该疾病的新途径。在本研究中，我们利用miRNA芯片研究了PDAC患者和健康对照者血清和肿瘤样本中的microRNA （miRNA/miR）谱，并确定了miR-1表达在PDAC中的潜在作用。共有43名在长治市人民医院就诊的诊断为PDAC的患者被邀请参加。采集血液和手术肿瘤样本，通过miRNA芯片和逆转录-定量聚合酶链反应（RT-qPCR）进行分析。此外，通过原位杂交（ISH）测定手术肿瘤组织的miRNAs状态。微阵列检测结果显示：i) PDAC患者血清中27个mirna和肿瘤组织中23个mirna与对照组相比存在差异；ii) miR-1、miR-10b和miR-214在PDAC组血清或肿瘤组织样本中均发生显著改变。RT-qPCR检测了PDAC患者的miRNA水平，结果证实了miRNA微阵列获得的结果。特别是，本研究的结果显示PDAC组织中miR-1的降低和miR-214的升高与PDAC患者的临床病理特征和生存率相关。本研究结果表明，mirna在PDAC的癌变过程中起着重要作用，并提供了有用的标志物，包括miR-1、miR-214和miR-10b，用于通过无创方法确定PDAC的预后。
Pancreatic ductal adenocarcinoma (PDAC) is a devastating malignant disease with a poor prognosis. PDAC is known to be difficult to diagnose at an early stage and to exhibit poor recurrence-free prognosis, but there is also a lack of effective treatment and limited knowledge of its biological characteristics. Therefore, there is an urgent requirement for an improved understanding of the cellular or molecular properties associated with PDAC, and to explore novel avenues for the diagnosis and treatment of this disease. In the present study, the microRNA (miRNA/miR) profiles of sera and tumor samples from patients with PDAC and healthy controls were investigated by miRNA microarray, and the potential role of miR-1 expression in PDAC was determined. A total of 43 patients attending the clinic diagnosed with PDAC at Changzhi City People's Hospital were invited to participate. Blood and surgical tumor samples were obtained for analysis by miRNA microarray and the reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The surgical tumor tissue was additionally used to determine miRNAs status by in situ hybridization (ISH). The results of microarray revealed that: i) 27 miRNAs in the sera and 23 miRNAs in the tumor tissues obtained from patients with PDAC were different compared with their matched controls; ii) miR-1, miR-10b and miR-214 were significantly altered in the PDAC group, either in the sera or tumor tissue samples. Results from the RT-qPCR, which detected the levels of miRNAs in patients with PDAC, confirmed those obtained from the miRNA microarray. In particular, the results of the present study revealed that decreased miR-1 and increased miR-214 in the PDAC tissues were associated with the clinicopathological features and survival rates of patients with PDAC. The results of the present study indicated that miRNAs serve an important role in PDAC carcinogenic progression and supplied useful markers, including miR-1, miR-214 and miR-10b, for determining PDAC prognosis using noninvasive methods.
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影响因子：
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作者：
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影响因子：
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作者：
Hu T;Chang YF;Xiao Z;Mao R;Tong J;Chen B;Liu GC;Hong Y;Chen HL;Kong SY;Huang YM;Xiyang YB;Jin H
通讯作者：
Jin H
DOI：
10.1016/j.ijbiomac.2015.08.016
发表时间：
2015-11-01
影响因子：
8.2
作者：
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通讯作者：
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