Combination of nanoindentation and quantitative backscattered electron imaging revealed altered bone material properties associated with femoral neck fragility.
Combination of nanoindentation and quantitative backscattered electron imaging revealed altered bone material properties associated with femoral neck fragility.
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DOI：
10.1007/s00223-009-9289-8
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发表时间：
2009-10
影响因子：
4.2
通讯作者：
Fratzl, P.
中科院分区：
文献类型：
--
作者：
Fratzl-Zelman, N.;Roschger, P.;Gourrier, A.;Weber, M.;Misof, B. M.;Loveridge, N.;Reeve, J.;Klaushofer, K.;Fratzl, P.
关键词：
假设骨质疏松性脆性骨折与骨材料性质的变化有关，而不仅仅是骨量减少。我们研究了5例女性髋关节骨折患者（74-92岁）和5例非骨折对照者（75-88岁）的整个股骨颈切片的上级和下部的皮质骨。分别使用定量背散射电子成像（qBEI）和扫描小角X射线散射在上级和低级区域的大面积中绘制典型钙含量（CaPeak）和矿物颗粒厚度参数（T）。此外，在局部水平上将压痕模量（E）和硬度（H）（通过纳米压痕测定）与纳米位置处的矿物质含量（CaInd）（从qBEI获得）进行比较。与下方区域相比，上方区域的CaPeak（− 2.2%，P = 0.002）、CaInd（− 1.8%，P = 0.048）、E（− 5.6%，P = 0.040）和H（− 6.0%，P = 0.016）显著较低。有趣的是，与对照组相比，骨折病例的CaPeak和CaInd也较低（分别为-2.6%，P = 0.006和-3.7%，P = 0.002），而E和H没有显示任何显著降低。T值在正常范围内，与区域（P = 0.181）或骨折状态（P = 0.551）无关。总之，观察到的股骨颈脆性似乎与矿物质含量降低相关，而不伴有骨材料刚度和硬度降低。该初步研究表明，有机基质成分中的硬化过程导致骨脆性，与矿物质含量无关。
Osteoporotic fragility fractures were hypothesized to be related to changes in bone material properties and not solely to reduction in bone mass. We studied cortical bone from the superior and inferior sectors of whole femoral neck sections from five female osteoporotic hip fracture cases (74–92 years) and five nonfractured controls (75–88 years). The typical calcium content (CaPeak) and the mineral particle thickness parameter (T) were mapped in large areas of the superior and inferior regions using quantitative backscattered electron imaging (qBEI) and scanning small-angle X-ray scattering, respectively. Additionally, indentation modulus (E) and hardness (H) (determined by nanoindentation) were compared at the local level to the mineral content (CaInd) at the indent positions (obtained from qBEI). CaPeak (−2.2%, P = 0.002), CaInd (−1.8%, P = 0.048), E (−5.6%, P = 0.040), and H (−6.0%, P = 0.016) were significantly lower for the superior compared to the inferior region. Interestingly, CaPeak as well as CaInd were also lower (−2.6%, P = 0.006, and –3.7%, P = 0.002, respectively) in fracture cases compared to controls, while E and H did not show any significant reduction. T values were in the normal range, independent of region (P = 0.181) or fracture status (P = 0.551). In conclusion, it appears that the observed femoral neck fragility is associated with a reduced mineral content, which was not accompanied by a reduction in stiffness and hardness of the bone material. This pilot study suggests that a stiffening process in the organic matrix component contributes to bone fragility independently of mineral content.
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影响因子：
4.3
作者：
Currey, John D
通讯作者：
Currey, John D
影响因子：
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作者：
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