Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene.

Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene.
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酿酒酵母 CDC6 基因的克隆和表征。

DOI:
10.1093/nar/16.24.11507
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发表时间:
1988
影响因子:
14.9
通讯作者:
H. Küntzel
H. Küntzel
中科院分区:
生物学2区
文献类型:
--
作者:
J. Lisziewicz;A. Godány;D. Agoston;H. Küntzel

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The yeast cell division cycle gene CDC6 was isolated by complementation of a temperature-sensitive cdc6 mutant with a genomic library. The amino acid sequence of the 48 kDalton CDC6 gene product, as deduced from DNA sequence data, includes the three consensus peptide motifs involved in guanine nucleotide binding and GTPase activity, a target site for cAMP-dependent protein kinase and a carboxy-terminal domain related to metallothionein sequences. A plasmid-encoded CDC6-beta-galactosidase hybrid protein was located at the plasma membrane by indirect immunofluorescence. Disruption experiments indicate that the CDC6 gene product is essential for mitotic growth.
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发表时间: 1987
影响因子: 5.6
作者:
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通讯作者: Byers,B
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发表时间: 1983
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发表时间: 1987-04-01
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作者:
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发表时间: 1988-02-19
期刊: SCIENCE
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