Exogenous melatonin reduces the inhibitory effect of osmotic stress on antioxidant properties and cell ultrastructure at germination stage of soybean.
Exogenous melatonin reduces the inhibitory effect of osmotic stress on antioxidant properties and cell ultrastructure at germination stage of soybean.
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外源褪黑素降低渗透胁迫对大豆萌发期抗氧化性能和细胞超微结构的抑制作用
DOI：
10.1371/journal.pone.0243537
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zhang M;He S;Qin B;Jin X;Wang M;Ren C;Cao L;Zhang Y
了解外源褪黑激素与水分亏缺胁迫的关系，对于缓解大豆（Glycine max（L.）梅里尔）在农业方面。研究了外源褪黑激素对聚乙二醇（PEG）6000诱导的水分亏缺胁迫下大豆抗氧化特性和细胞超微结构的影响。以干旱敏感型大豆品种绥农26为材料，研究了不同浓度褪黑激素（0、300、500 μmol·L-1）对干旱胁迫（PEG-6000：3%和6%）下大豆种子的影响。结果表明，PEG胁迫对大豆种子的发芽率、发芽势、发芽指数和胚根形状均有不同程度的影响。此外，不同浓度的PEG胁迫导致细胞内活性氧（H2 O2，O2·−）含量过高，导致脂质膜过氧化，如电解质渗漏（EL）和丙二醛（MDA）含量增加，从而导致细胞完整性受损。而褪黑激素浸种后，细胞膜脂质过氧化程度降低，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）等抗氧化酶活性进一步提高，减少了ROS的过量产生。可溶性蛋白质和脯氨酸也得到了类似的结果，这可能有助于调节渗透压和维持细胞的完整性。在这些酶的相互作用下，500 μ mol·L-1褪黑素较300 μmol·L-1褪黑素能更有效地清除ROS，降低细胞过氧化反应。总的来说，500 μmol·L-1褪黑素的效果优于300 μmol·L-1。可见，褪黑激素浸种对水分胁迫下大豆种子的萌发有促进作用。
Understanding the relationship between exogenous melatonin and water deficit stress is crucial for alleviating the effects of water deficit stress at germination stage of soybean (Glycine max (L.) Merrill) in agriculture. This study investigated the effects of exogenous melatonin on soybean antioxidant properties and cell ultrastructure under water deficit stress induced by polyethylene glycol (PEG) 6000. The drought-sensitive soybean variety Suinong 26 was used as the material to study the effects of different concentrations of melatonin (0, 300, 500 μmol·L-1) soaking soybean seeds under drought stress (PEG-6000: 3% and 6%). The results showed that the germination rate (GR), germination potential (GP), germination index (GI) and radicle shape of soybean were affected negatively to different degrees under PEG stress. Moreover, stress induced by different PEG concentrations overproduced the content of reactive oxygen species (H2O2, O2·−) in cells, leading to increased lipid membrane peroxidation as electrolyte leakage (EL) and malondialdehyde (MDA) content, which resulted in impaired cell integrity. However, after seeds soaking with melatonin, the lipid peroxidation of the cell membrane was reduced, and the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) further increased to minimize the excessive generation of ROS. Similar results were obtained for soluble protein and proline, that may help in regulating the osmotic pressure and maintain cellular integrity. With the interaction of these enzymes, compared with 300 μmol·L-1 melatonin, 500 μmol·L-1 melatonin could more effective to remove the ROS and reduce cell peroxidation. Overall, 500 μmol·L-1 melatonin performed better than 300 μmol·L-1. In conclusion, the seed soaking with melatonin promoted the germination of soybean seeds under water stress.
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