Antiviral activity of human OASL protein is mediated by enhancing signaling of the RIG-I RNA sensor.
Antiviral activity of human OASL protein is mediated by enhancing signaling of the RIG-I RNA sensor.
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DOI：
10.1016/j.immuni.2014.05.007
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发表时间：
2014-06-19
期刊：
影响因子：
32.4
通讯作者：
Sarkar, Saumendra N.
中科院分区：
文献类型：
--
作者：
Zhu, Jianzhong;Zhang, Yugen;Ghosh, Arundhati;Cuevas, Rolando A.;Forero, Adriana;Dhar, Jayeeta;Ibsen, Mikkel Soes;Schmid-Burgk, Jonathan Leo;Schmidt, Tobias;Ganapathiraju, Madhavi K.;Fujita, Takashi;Hartmann, Rune;Barik, Sailen;Hornung, Veit;Coyne, Carolyn B.;Sarkar, Saumendra N.
病毒感染是通过维甲酸诱导基因I(RIG-I，也称为DDX58)在细胞质中感应的，它需要RNA和多聚泛素结合来诱导I型干扰素(IFN)，并激活细胞天然免疫。我们发现，人干扰素诱导的寡腺苷合成酶样蛋白(OASL)具有抗病毒活性，并通过模拟多聚泛素介导RIG-I的激活。OASL表达的缺失降低了RIG-I信号转导，增强了病毒在人类细胞中的复制。相反，OASL的表达以rig-I依赖的方式抑制了一些病毒的复制，并增强了rig-I介导的干扰素的诱导。OASL与RIG-I相互作用和共定位，并通过其C端泛素样结构域特异性增强RIG-I信号。来自Oasl2基因缺陷小鼠的骨髓来源的巨噬细胞表明，在两个人OASL的小鼠同源基因中，Oasl2在功能上与人OASL相似。我们的发现表明，人类OASL通过增强RIG-I激活来促进宿主抗病毒反应的机制。
Virus infection is sensed in the cytoplasm by retinoic acid-inducible gene I (RIG-I, also known as DDX58), which requires RNA and polyubiquitin binding to induce type I interferon (IFN), and activate cellular innate immunity. We show that the human IFN-inducible oligoadenylate synthetases-like (OASL) protein had antiviral activity and mediated RIG-I activation by mimicking polyubiquitin. Loss of OASL expression reduced RIG-I signaling and enhanced virus replication in human cells. Conversely, OASL expression suppressed replication of a number of viruses in a RIG-I-dependent manner and enhanced RIG-I-mediated IFN induction. OASL interacted and colocalized with RIG-I, and through its C-terminal ubiquitin-like domain specifically enhanced RIG-I signaling. Bone marrow derived macrophages from mice deficient for Oasl2 showed that among the two mouse orthologs of human OASL; Oasl2 is functionally similar to human OASL. Our findings show a mechanism by which human OASL contributes to host antiviral responses by enhancing RIG-I activation.
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RIG-I 样受体的免疫信号传导。
影响因子：
32.4
作者：
Loo YM;Gale M Jr
通讯作者：
Gale M Jr
影响因子：
64.8
作者：
Jiang, Fuguo;Ramanathan, Anand;Miller, Matthew T.;Tang, Guo-Qing;Gale, Michael, Jr.;Patel, Smita S.;Marcotrigiano, Joseph
通讯作者：
Marcotrigiano, Joseph
DOI：
10.1126/science.1244040
发表时间：
2013-09-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li XD;Wu J;Gao D;Wang H;Sun L;Chen ZJ
通讯作者：
Chen ZJ
影响因子：
44.1
作者：
Goswami, Ramansu;Majumdar, Tanmay;Barik, Sailen
通讯作者：
Barik, Sailen
DOI：
10.1046/j.1432-1327.1998.2570319.x
发表时间：
1998-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Rebouillat, D;Marié, I;Hovanessian, AG
通讯作者：
Hovanessian, AG