Oral vaccination with recombinant Lactobacillus casei expressing Aha1 fused with CTB as an adjuvant against Aeromonas veronii in common carp (Cyprinus carpio).
Oral vaccination with recombinant Lactobacillus casei expressing Aha1 fused with CTB as an adjuvant against Aeromonas veronii in common carp (Cyprinus carpio).
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使用表达 Aha1 的重组干酪乳杆菌与 CTB 融合作为佐剂针对鲤鱼（Cyprinus carpio）中的维氏气单胞菌进行口服疫苗接种
DOI：
10.1186/s12934-022-01839-9
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发表时间：
2022-06-13
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
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作者：
韦氏气单胞菌(Aeromonas veronii，A.veronii)是一种能感染人、动物和水生生物的病原菌，对鲤鱼等水生生物的健康构成巨大威胁。本研究以干酪乳杆菌CC16为抗原递送载体，与霍乱毒素B亚单位(CTB)融合，分别构建了重组干酪乳杆菌ppg-Aha1/LC CC16(表面展示)和ppg-Aha1-CTB/LC CC16(表面展示)。并对鲤鱼经口免疫应答进行了探讨。结果表明，重组菌株能刺激高水平的血清特异性抗体免疫球蛋白M(IgM)，诱导鲤鱼的酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、补体C3、C4、溶菌酶(LZM)、凝集素和超氧化物歧化酶(SOD)活性高于对照组。同时，与LC-PPG或PBS组相比，IL-10、IL-1β、肿瘤坏死因子-β、免疫球蛋白Z1和免疫球蛋白Z2的表达显著上调，提示体液免疫和细胞免疫反应被触发。此外，重组干酪乳杆菌还能在鱼肠道内存活和定植。重组干酪乳杆菌对白纹伊蚊具有免疫保护作用，与对照组相比，pPG-AHA1-CTB/LC CC16(64.29%)和ppg-AHA1/LC CC16(53.57%)具有更高的存活率。因此，我们认为重组ppg-AHA1/LC CC16和ppg-AHA1-CTB/LC CC16可能是研制Veronii口服疫苗的良好策略。网上版载有补充材料，可在10.1186/s12934-022-01839-9查阅。
Aeromonas veronii (A. veronii) is a pathogenic that can infect human, animal and aquatic organisms, in which poses a huge threat to the health of many aquatic organisms such as Cyprinus carpio. In this study, Lactobacillus casei (L. casei) strain CC16 was used as antigen deliver carrier and fused with cholera toxin B subunit (CTB) as an adjuvant to construct the recombinant L. casei pPG-Aha1/Lc CC16(surface-displayed) and pPG-Aha1-CTB/Lc CC16(surface-displayed) expressing Aha1 protein of A. veronii, respectively. And the immune responses in Cyprinus carpio by oral route was explored. Our results demonstrated that the recombinant strains could stimulate high serum specific antibody immunoglobulin M (IgM) and induce a stronger acid phosphatase (ACP), alkaline phosphatase (AKP), C3, C4, lysozyme (LZM), Lectin and superoxide dismutase (SOD) activity in Cyprinus carpio compared with control groups. Meanwhile, the expression of Interleukin-10 (IL-10), Interleukin-1β (IL-1β), Tumor Necrosis Factor-α (TNF-α), immunoglobulin Z1 (IgZ1) and immunoglobulin Z2 (IgZ2) in the tissues were significantly upregulated compared with Lc-pPG or PBS groups, indicating that humoral and cell immune response were triggered. Additionally, recombinant L. casei could survive and colonize in fish intestine. Significantly, recombinant L. casei provides immune protection against A. veronii infection, which Cyprinus carpio received pPG-Aha1-CTB/Lc CC16 (64.29%) and pPG-Aha1/Lc CC16 (53.57%) had higher survival rates compared with the controls. Thus, we demonstrated that recombinant pPG-Aha1/Lc CC16 and pPG-Aha1-CTB/Lc CC16 may be the promising strategy for the development of an oral vaccine against A. veronii. The online version contains supplementary material available at 10.1186/s12934-022-01839-9.
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影响因子：
5.5
作者：
Gao, Shanshan;Zhao, Na;Zhao, Baohua
通讯作者：
Zhao, Baohua
DOI：
10.3390/pathogens9121031
发表时间：
2020-12-09
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Hatrongjit R;Kerdsin A;Takeuchi D;Wongsurawat T;Jenjaroenpun P;Chopjitt P;Boueroy P;Akeda Y;Hamada S
通讯作者：
Hamada S
影响因子：
1
作者：
Harandi, Ali M.;Medaglini, Donata
通讯作者：
Medaglini, Donata
影响因子：
4.7
作者：
Guardiola, Francisco A.;Cuesta, Alberto;Esteban, Maria A.
通讯作者：
Esteban, Maria A.
影响因子：
4.1
作者：
Kang, Yuanhuan;Pan, Xiaoyi;Qian, Aidong
通讯作者：
Qian, Aidong