Host ABCE1 is at plasma membrane HIV assembly sites and its dissociation from Gag is linked to subsequent events of virus production.
Host ABCE1 is at plasma membrane HIV assembly sites and its dissociation from Gag is linked to subsequent events of virus production.
复制标题
宿主 ABCE1 位于质膜 HIV 组装位点，其与 Gag 的解离与随后的病毒产生事件相关。
DOI：
10.1111/j.1600-0854.2006.00524.x
复制
发表时间：
2007-03
期刊：
影响因子：
4.5
通讯作者：
Lingappa, Jaisri R.
中科院分区：
文献类型：
--
作者：
Dooher, Julia E.;Schneider, Bobbie L.;Reed, Jonathan C.;Lingappa, Jaisri R.
在灵长类细胞中，单个HIV-1衣壳的组装涉及数千个Gag多肽的多聚体，通常在质膜上。尽管研究支持HIV-1通过含有GAG和细胞内三磷酸腺苷酶ABCE1(也称为HP68或核糖核酸酶L抑制剂)的复合体进行组装的模型，但这些复合体是否构成真正的组装中间体仍然存在争议。在这里，我们通过在灵长类细胞中进行脉冲标记来证明，在翻译后的几分钟内，GAG群体与内源性ABCE1相关。在接下来的∼2小时内，GAG-ABCE1复合体的尺寸增大到接近未成熟衣壳的尺寸。在病毒粒子成熟过程中，ABCE1与GAG的解离密切相关，当HIV-1蛋白酶失活时，ABCE1的解离效率要低得多。最后，定量双标记免疫金电子显微镜显示，ABCE1被招募到质膜上野生型Gag的组装部位，而不是质膜上组装缺陷的Gag突变体的部位。综上所述，这些发现表明，在整个衣壳形成过程中，存在于组装的质膜位置的一群Gag与ABCE1相关，直到病毒成熟，然后病毒释放。此外，数据表明，Gag-ABCE1解离和随后的病毒粒子产生事件之间存在联系。
In primate cells, assembly of a single HIV-1 capsid involves multimerization of thousands of Gag polypeptides, typically at the plasma membrane. Although studies support a model in which HIV-1 assembly proceeds through complexes containing Gag and the cellular adenosine triphosphatase ABCE1 (also termed HP68 or ribonuclease L inhibitor), whether these complexes constitute true assembly intermediates remains controversial. Here we demonstrate by pulse labeling in primate cells that a population of Gag associates with endogenous ABCE1 within minutes of translation. In the next ∼2 h, Gag–ABCE1 complexes increase in size to approximately that of immature capsids. Dissociation of ABCE1 from Gag correlates closely with Gag processing during virion maturation and occurs much less efficiently when the HIV-1 protease is inactivated. Finally, quantitative double-label immunogold electron microscopy reveals that ABCE1 is recruited to sites of assembling wild-type Gag at the plasma membrane but not to sites of an assembly-defective Gag mutant at the plasma membrane. Together these findings demonstrate that a population of Gag present at plasma membrane sites of assembly associates with ABCE1 throughout capsid formation until the onset of virus maturation, which is then followed by virus release. Moreover, the data suggest a linkage between Gag–ABCE1 dissociation and subsequent events of virion production.
登录
查看更多内容
影响因子：
5.4
作者：
Halwani, R;Khorchid, A;Kleiman, L
通讯作者：
Kleiman, L
影响因子：
4.8
作者：
Chen, ZQ;Dong, JS;Dean, M
通讯作者：
Dean, M
在无细胞系统中组装人类免疫缺陷病毒衣壳的多步，依赖于ATP的途径。
DOI：
10.1083/jcb.136.3.567
发表时间：
1997-02-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lingappa JR;Hill RL;Wong ML;Hegde RS
通讯作者：
Hegde RS
影响因子：
5.4
作者：
Campbell, S;Vogt, VM
通讯作者：
Vogt, VM
DOI：
10.1073/pnas.191224698
发表时间：
2001-09-11
影响因子：
11.1
作者：
Campbell, S;Fisher, RJ;Rein, A
通讯作者：
Rein, A