Characterization of IncC Plasmids in Enterobacterales of Food-Producing Animals Originating From China.
Characterization of IncC Plasmids in Enterobacterales of Food-Producing Animals Originating From China.
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中国食品动物肠杆菌中 IncC 质粒的鉴定
DOI：
10.3389/fmicb.2020.580960
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang HN
中科院分区：
文献类型：
--
作者：
Zhang Y;Lei CW;Chen X;Yao TG;Yu JW;Hu WL;Mao X;Wang HN
不相容组C （IncC）质粒因其广泛的宿主范围和含有关键的抗生素耐药基因而受到关注。由于这些抗性基因可以从食用动物传播给人类，因此这些质粒的扩散构成了公共卫生风险。本研究从中国食用动物中收集了20个IncC质粒，并通过Oxford Nanopore Technologies的长读测序对其进行了表征。根据IncC主干的4个关键差异，将4个IncC质粒分类为1型，15个为1/2型杂交，1个为2型。根据1型和2型IncC主干同源重组事件不同导致的序列差异，将15个1/2型杂种进一步分为13个1/2a型和2个1/2b型。辅助耐药模块的基因组比较表明，不同的IncC质粒通过不同模块的损失和获得表现出不同的表型，主要在携带blacmy的区域，以及两个抗生素耐药岛称为ARI-A和ARI-B。有趣的是，除了插入和删除事件外，在4个type /2a质粒的IncC基因组中还发现了IS26或is1294介导的大序列倒置，这表明插入序列介导的重排也促进了IncC基因组的多样性。本研究对从中国食用动物中鉴定出的IncC质粒的结构多样化和多药耐药性提供了深入的了解。
Incompatibility group C (IncC) plasmids have received attention due to their broad host range and because they harbor key antibiotic resistance genes. Because these resistance genes can spread from food-producing animals to human, the proliferation of these plasmids represents a public health risk. In this study, a total of 20 IncC plasmids were collected from food-producing animals in China, and characterized by Oxford Nanopore Technologies long-read sequencing. Based on four key differences of the IncC backbone, 4 IncC plasmids were classified as type 1, 15 were classified as type 1/2 hybrid, and one was classified as type 2. The 15 type 1/2 hybrids were further divided into 13 type 1/2a and 2 type 1/2b, based on sequence differences arising from different homologous recombination events between type 1 and type 2 IncC backbones. Genome comparison of accessory resistance modules showed that different IncC plasmids exhibited various phenotypes via loss and gain of diverse modules, mainly within the blaCMY-carrying region, and two antibiotic resistance islands designated ARI-A and ARI-B. Interestingly, in addition to insertion and deletion events, IS26 or IS1294-mediated large sequence inversions were found in the IncC genome of the 4 type1/2a plasmids, suggesting that insertion sequence-mediated rearrangements also promote the diversity of the IncC genome. This study provides insight into the structural diversification and multidrug resistance of IncC plasmids identified from food-producing animals in China.
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影响因子：
2.6
作者：
Ambrose, Stephanie J.;Harmer, Christopher J.;Hall, Ruth M.
通讯作者：
Hall, Ruth M.
影响因子：
2.6
作者：
Harmer, Christopher J.;Hall, Ruth M.
通讯作者：
Hall, Ruth M.
影响因子：
5.2
作者：
Cheng, Qiaoxiang;Jiang, Xiaoyuan;Dai, Erhei
通讯作者：
Dai, Erhei
DOI：
10.1099/00221287-81-1-171
发表时间：
1974-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HEDGES, RW
通讯作者：
HEDGES, RW
影响因子：
3.7
作者：
Kaas RS;Leekitcharoenphon P;Aarestrup FM;Lund O
通讯作者：
Lund O