Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance.
Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance.
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DOI：
10.1093/jxb/eru003
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发表时间：
2014-04
影响因子：
6.9
通讯作者：
Kater MM
中科院分区：
文献类型：
--
作者：
Simonini S;Kater MM
BPC因子是发育过程的重要调节因子。在此，我们将BPC在控制分生组织大小中的作用归于BPC，将它们描述为几个同源盒基因的直接调节子。碱性五半胱氨酸（BCP）家族是GAGA结合蛋白的一个特征不明确的植物转录因子家族。在拟南芥中，有七个成员（BPC 1 -7）广泛表达，它们可以潜在地结合超过3000个拟南芥GAGA重复序列基因。迄今为止，已知BPC是INNER NO OUTER（INO）、SEEDSTICK（STK）和LEAFY COTYLEDON 2（LEC 2）基因的直接调节子。由于高功能冗余，单敲除或双bpc突变体组合都不会引起异常表型。bpc 1 -2 bpc 2 bpc 3三突变体表现出几个多效性的发育缺陷，包括花序分生组织的扩大和多生花器官的花。在这里，我们通过表达分析和染色质免疫沉淀试验证明，这种表型可能是由于SHOOTMERISTEMLESS（STM）和BREVIPEDICELLUS/KNAT 1（BP）基因表达的失调，这两个基因都是BPC的直接靶点。此外，我们指定BPC在分生组织中细胞分裂素含量的精细调节中起作用，因为异戊烯基转移酶7（IPT 7）和拟南芥反应调节因子7（ARR 7）基因在bpc 1 -2 bpc 2 bpc 3三重突变体中过表达。
BPC factors are important regulators of developmental processes. Here we assign a role to BPCs in the control of meristem size, characterizing them as direct regulators of several HOMEOBOX genes The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs are known to be direct regulators of the INNER NO OUTER (INO), SEEDSTICK (STK), and LEAFY COTYLEDON 2 (LEC2) genes. Because of the high functional redundancy, neither single knockout nor double bpc mutant combinations cause aberrant phenotypes. The bpc1-2 bpc2 bpc3 triple mutant shows several pleiotropic developmental defects, including enlargement of the inflorescence meristem and flowers with supernumerary floral organs. Here, we demonstrated through expression analysis and chromatin immunoprecipitation assays that this phenotype is probably due to deregulation of the expression of the SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS/KNAT1 (BP) genes, which are both direct targets of BPCs. Moreover, we assigned a role to BPCs in the fine regulation of the cytokinin content in the meristem, as both ISOPENTENYLTRANSFERASE 7 (IPT7) and ARABIDOPSIS RESPONSE REGULATOR 7 (ARR7) genes were shown to be overexpressed in the bpc1-2 bpc2 bpc3 triple mutant.
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影响因子：
11.6
作者：
Dreni, Ludovico;Pilatone, Alessandro;Kater, Martin M.
通讯作者：
Kater, Martin M.
影响因子：
11.6
作者：
Brambilla, Vittoria;Battaglia, Raffaella;Colombo, Lucia
通讯作者：
Colombo, Lucia
影响因子：
7.2
作者：
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通讯作者：
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影响因子：
2.6
作者：
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通讯作者：
Kater, Martin M.
影响因子：
7.4
作者：
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通讯作者：
Schmuelling, Thomas