Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment.

Production of a novel bioflocculant MNXY1 by Klebsiella pneumoniae strain NY1 and application in precipitation of cyanobacteria and municipal wastewater treatment.
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DOI:
10.1111/j.1365-2672.2011.05080.x
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发表时间:
2011-09
影响因子:
4
通讯作者:
Wang Z
Wang Z
中科院分区:
生物学3区
文献类型:
--
作者:
Nie M;Yin X;Jia J;Wang Y;Liu S;Shen Q;Li P;Wang Z

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目的:分离、鉴定新型微生物絮凝剂产生菌,优化微生物絮凝剂的生产工艺,并对其应用前景进行评价。在化学成分确定的培养基上筛选出一株产生新型生物絮凝剂(MNXY 1)的肺炎克雷伯氏菌NY1。根据16S rRNA基因序列、形态特征和显微特征对该菌进行了分类。MNXY1蛋白质含量为26%,总糖含量为66%。NMR分析显示,MNXY1的糖单体组成为葡萄糖、半乳糖和奎诺糖。确定了MNXY 1生产的有利培养条件。菌株NY1产生高水平(14.9 g l−1)的MNXY 1。MNXY1是热稳定和耐受极端pH值。它沉淀了54%的蓝藻从实验室培养和72%的总悬浮固体从原废水。经鉴定,菌株NY1产一种新型絮凝剂MNXY 1。MNXY 1在实际应用中的优异性能及其大量的可用性使其具有进一步研究和开发工业规模应用的吸引力。
To isolate and characterize the novel bioflocculant-producing bacteria, to optimize the bioflocculant production and evaluate its potential applications. Klebsiella pneumoniae strain NY1, a bacterium that produces a novel bioflocculant (MNXY1), was selected on the chemically defined media. It was classified according to the 16S rRNA gene sequence, morphological and microscopic characteristics. MNXY1 was characterized to contain 26% protein and 66% total sugar. The constituent sugar monomers of MNXY1, revealed by NMR analysis, are glucose, galactose and quinovose. Favorable culture conditions for MNXY1 production were determined. Strain NY1 produces a high level (14.9 g l−1) of MNXY1. MNXY1 is thermostable and tolerant to the extreme pH. It precipitated 54% of cyanobacteria from laboratory culture and 72% of the total suspended solids from raw wastewater. Strain NY1 was identified to produce a novel bioflocculant MNXY1. The outstanding performance of MNXY1 in practical applications and its availability in copious amounts make it attractive for further investigation and development for industrial scale applications.
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