Expression of fatty acid and lipid biosynthetic genes in developing endosperm of Jatropha curcas.
Expression of fatty acid and lipid biosynthetic genes in developing endosperm of Jatropha curcas.
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麻疯树胚乳发育中脂肪酸和脂质生物合成基因的表达。
DOI：
10.1186/1754-6834-5-47
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发表时间：
2012-07-18
影响因子：
6.3
通讯作者：
Yin Z
中科院分区：
文献类型：
--
作者：
Gu K;Yi C;Tian D;Sangha JS;Hong Y;Yin Z
油料植物种子中脂肪酸和脂质生物合成基因的时空表达与储存脂质的积累有关。麻疯树（jatropha curcas L.）是一种潜在的生物燃料植物，其储存脂质主要在种子胚乳中合成和积累。虽然麻疯树中脂肪酸和脂质生物合成基因已被鉴定，但这些基因在胚乳不同发育阶段的表达尚未被系统研究。透射电镜研究发现，麻疯树种子发育胚乳油体形成最早出现在受精后28天，42天开始活跃发育，56天达到最大数量和最大尺寸。从麻疯树发育胚乳的标准化cDNA文库中鉴定出68个参与脂肪酸和脂质生物合成的酶、蛋白或亚基编码基因。基因表达的定量反转录聚合酶链反应分析表明，根据基因在胚乳发育过程中的相对表达模式，68个基因可分为5类。第一类共有47个基因，呈钟形表达模式，在28和42 DAF时表达高峰，在14和56 DAF时表达低。第二类包含8个基因，8个基因的表达量从14 DAF不断增加到56 DAF。第三类包括2个基因，这两个基因在胚乳发育过程中均有组成性表达。第IV类共有9个基因，14和28 DAF时表达量高，42 ~ 56 DAF时表达量下降。第V类由2个基因组成，两个基因在14 DAF时表达量中等，在28或42 DAF时表达量最低，在56 DAF时表达量最高。此外，在胚乳发育过程中，编码类似功能的酶或蛋白质的基因也存在差异表达。麻疯树胚乳油体的形成受发育调控。麻疯树种子中大多数脂肪酸和脂质生物合成基因的表达与油体和胚乳的发育高度一致，而编码功能相似的酶的基因在胚乳发育过程中可能存在差异表达。这些结果不仅为麻疯树发育胚乳中脂肪酸和脂质生物合成基因的时空表达提供了初步信息，而且对确定种子发育过程中储存脂质生物合成和积累的限速基因具有重要价值。
Temporal and spatial expression of fatty acid and lipid biosynthetic genes are associated with the accumulation of storage lipids in the seeds of oil plants. In jatropha (Jatropha curcas L.), a potential biofuel plant, the storage lipids are mainly synthesized and accumulated in the endosperm of seeds. Although the fatty acid and lipid biosynthetic genes in jatropha have been identified, the expression of these genes at different developing stages of endosperm has not been systemically investigated. Transmission electron microscopy study revealed that the oil body formation in developing endosperm of jatropha seeds initially appeared at 28 days after fertilization (DAF), was actively developed at 42 DAF and reached to the maximum number and size at 56 DAF. Sixty-eight genes that encode enzymes, proteins or their subunits involved in fatty acid and lipid biosynthesis were identified from a normalized cDNA library of jatropha developing endosperm. Gene expression with quantitative reverse-transcription polymerase chain reaction analysis demonstrated that the 68 genes could be collectively grouped into five categories based on the patterns of relative expression of the genes during endosperm development. Category I has 47 genes and they displayed a bell-shaped expression pattern with the peak expression at 28 or 42 DAF, but low expression at 14 and 56 DAF. Category II contains 8 genes and expression of the 8 genes was constantly increased from 14 to 56 DAF. Category III comprises of 2 genes and both genes were constitutively expressed throughout endosperm development. Category IV has 9 genes and they showed a high expression at 14 and 28 DAF, but a decreased expression from 42 to 56 DAF. Category V consists of 2 genes and both genes showed a medium expression at 14 DAF, the lowest expression at 28 or 42 DAF, and the highest expression at 56 DAF. In addition, genes encoding enzymes or proteins with similar function were differentially expressed during endosperm development. The formation of oil bodies in jatropha endosperm is developmentally regulated. The expression of the majority of fatty acid and lipid biosynthetic genes is highly consistent with the development of oil bodies and endosperm in jatropha seeds, while the genes encoding enzymes with similar function may be differentially expressed during endosperm development. These results not only provide the initial information on spatial and temporal expression of fatty acid and lipid biosynthetic genes in jatropha developing endosperm, but are also valuable to identify the rate-limiting genes for storage lipid biosynthesis and accumulation during seed development.
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影响因子：
1.8
作者：
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通讯作者：
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