Visualization of supramolecular structure of Pluronic F127 micellar hydrogels using cryo-TEM.
Visualization of supramolecular structure of Pluronic F127 micellar hydrogels using cryo-TEM.
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DOI：
10.1016/j.mex.2020.101084
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发表时间：
2020
期刊：
影响因子：
1.9
通讯作者：
de Farias MA
中科院分区：
文献类型：
--
作者：
da Silva LCE;Borges AC;de Oliveira MG;de Farias MA
水凝胶中的单个Pluronic® F127胶束的可视化。胶束水凝胶的超分子结构演化与浓度的关系。高粘度溶液的急冻。Pluronic® F127胶束水凝胶由于其作为药物递送系统的多功能性而越来越受到生物医学领域的关注。Pluronic® F127是一种对称的两亲性三嵌段共聚物，它在水介质中自组装成胶束，随着温度或浓度的升高而聚集在一起，形成不可流动的水凝胶。这些水凝胶的微观结构通常通过小角X射线散射来研究，这不是一种容易获得的技术。相反，低温TEM是一种广泛用于研究含水系统形态的技术。在Pluronic® F127胶束系统的情况下，升高的粘度对样品制备以及因此对低温TEM观察提出了重大挑战。在这里，我们展示了一个值得信赖的，廉价的和容易获得的方法，用于制备高粘度胶束溶液和非流动性水凝胶使用自动玻璃化系统的标本。通过这种方法，我们不仅能够可视化单个Pluronic® F127胶束的形态，还能够可视化作为浓度函数的超分子结构演变。这种方法为水凝胶表征提供了广泛的机会，尽管可能需要额外的系统研究，以便优化和复制类似系统。
Visualization of individual Pluronic® F127 micelles in hydrogels. Supramolecular structure evolution of micellar hydrogels as a function of concentration. Plunge-freezing of high viscosity solutions. Pluronic® F127 micellar hydrogels are of growing interest to the biomedical field due to their versatility as drug delivery systems. Pluronic® F127 is a symmetric and amphiphilic triblock copolymer which in aqueous medium self-assembles into micelles that pack togetherwith increasing temperature or concentration, leading to non-flowable hydrogels. The microstructure of these hydrogels is usually investigated by small-angle X-ray scattering, which is not a readily available technique. Conversely, cryo-TEM is a widespread technique used for investigating the morphology of aqueous systems. In the case of Pluronic® F127 micellar systems, the elevated viscosity poses a significant challenge for specimen preparation and, consequently, for cryo-TEM observation. Herein, we show a trustworthy, inexpensive and readily available methodology for preparing specimens of highly viscous micellar solutions and non-flowable hydrogels using an automated vitrification system. With this methodology we were able to visualize not only the morphology of individual Pluronic® F127 micelles -but also the supramolecular structure evolution as a function of concentration. This methodology opens up a wide range of opportunities for hydrogel characterization, although additional systematic studies might be required in order to optimize and replicate it for similar systems.
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影响因子：
9.9
作者：
Picheth, Guilherme F.;da Silva, Laura C. E.;de Oliveira, Marcelo G.
通讯作者：
de Oliveira, Marcelo G.
影响因子：
8.4
作者：
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通讯作者：
O'Reilly, Rachel K.
影响因子：
3.9
作者：
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通讯作者：
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影响因子：
5.5
作者：
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通讯作者：
Talmon, Y
影响因子：
3.3
作者：
Lam, YM;Grigorieff, N;Goldbeck-Wood, G
通讯作者：
Goldbeck-Wood, G