Poly I:C facilitates the phosphorylation of Ctenopharyngodon idellus type I IFN receptor subunits and JAK kinase
Poly I:C facilitates the phosphorylation of Ctenopharyngodon idellus type I IFN receptor subunits and JAK kinase
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Poly I:C 促进草鱼 I 型 IFN 受体亚基和 JAK 激酶的磷酸化
DOI：
10.1016/j.fsi.2016.10.042
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发表时间：
2017
影响因子：
4.7
通讯作者：
Chengyu Hu
中科院分区：
文献类型：
--
作者：
Qunhao Hou;Ruiyue Gong;Xiancheng Liu;Huiling Mao;Xiaowen Xu;Dan Liu;Zao Dai;Haizhou Wang;Binhua Wang;Chengyu Hu
JAK家族成员JAK 1和TYK 2参与干扰素介导的JAK-STAT信号通路。与哺乳动物类似，鱼类JAK 1和TYK 2也通过磷酸化细胞因子受体和STAT发挥其潜在的生物学活性。本研究克隆并鉴定了草鱼JAK 1（CiJAK 1）和TYK 2（CiTYK 2）基因。CiJAK 1（KT 724352.1）的cDNA全长为3829 bp，开放阅读框（ORF）为3465 bp，编码1154个氨基酸。CiTYK 2（KT 724353.1）全长cDNA为4337 bp，其中开放阅读框为3168 bp，编码1055个氨基酸。在结构上，两者均具有B41、SH 2、TyrKc和TyrKc的共同结构域，系统发育树分析表明，CiJAK 1和CiTYK 2与斑马鱼和鲤鱼的同源性较高。聚肌苷酸-聚胞苷酸（Poly I：C）是一种合成的dsRNA类似物，可以启动JAK-STAT抗病毒信号通路。为了阐明Poly I：C启动鱼类抗病毒信号通路的分子机制，用Poly I：C刺激CIK细胞，然后用10% SDS-PAGE对细胞裂解液进行分离。结果表明，Poly I：C不仅显著提高了CiJAK 1和CiTYK 2的表达水平，而且还诱导了CiJAK 1和CiTYK 2以及C. idellustypeIFN受体亚基CiCRFB 1和CiCRFB 5的磷酸化。结果表明，p-CiJAK 1和p-CiTYK 2在Poly I：C刺激后3 h明显上调，而CiCRFB 1和CiCRFB 5的磷酸化水平在Poly I：C刺激后12 h明显上调。作为JAK-STAT信号通路反馈调节的基本机制，CIK细胞中CiCRFB 1和CiCRFB 5的过表达促进了CiJAK 1和CiTYK 2的磷酸化。
Members of the Janus kinase (JAK) family, JAK1 and TYK2 take part in JAK-STAT signaling pathway mediated by interferon in mammalian cells. Similar to the mammalian counterparts, fish JAK1 and TYK2 also perform their potential biological activities by phosphorylating cytokine receptors and STAT. In the present study,Ctenopharyngodon idellus JAK1(CiJAK1) andTYK2(CiTYK2) were cloned and identified. The full-length cDNA ofCiJAK1(KT724352.1) is 3829 bp, with an Open Reading Frame (ORF) of 3465 bp encoding a putative protein of 1154 amino acids. The full-length cDNA ofCiTYK2(KT724353.1) is 4337 bp, including an ORF of 3168 bp encoding 1055 amino acids. Structurally, both of them have B41, SH2, TyrKc and TyrKc common domains.CiJAK1 andCiTYK2 share a high degree of homology with their respective counterparts fromDanio rerioandCyprinus carpioby phylogenetic tree analysis. Polyinosinic-polycytidylic acid (Poly I:C), a synthetic dsRNA analogue, can launch the JAK-STAT antiviral signaling pathway. To elucidate the molecular mechanism of Poly I:C initiating the antiviral signaling pathway in fish,C. idelluskidney (CIK) cells were stimulated with Poly I:C and then the cell lysates were separated on 10% SDS-PAGE. The results showed that not only Poly I:C drastically increased the expression level ofCiJAK1andCiTYK2, but also it induced the phosphorylation ofCiJAK1 andCiTYK2, as well asC. idellustype I IFN receptor subunits,CiCRFB1 andCiCRFB5. In detail, the levels ofp-CiJAK1 andp-CiTYK2 were evidently up-regulated at 3 h post stimulation; however the phosphorylation levels ofCiCRFB1 andCiCRFB5 displayed a sharp up-regulation at 12 h post stimulation of Poly I:C. As a basic mechnism of feedback regulation of JAK-STAT signaling pathway, overexpression ofCiCRFB1 andCiCRFB5 in CIK cells facilitated the phosphorylation ofCiJAK1 andCiTYK2.
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影响因子：
2.2
作者：
Liu, Ying;Zhang, Zhe;Chen, Wei
通讯作者：
Chen, Wei
甲基-CPG结合结构域蛋白的比较研究。
影响因子：
4.4
作者：
Roloff, TC;Ropers, HH;Nuber, UA
通讯作者：
Nuber, UA
影响因子：
4.8
作者：
Staerk, J;Kallin, A;Constantinescu, SN
通讯作者：
Constantinescu, SN
影响因子：
2.3
作者：
Ank, Nina;West, Hans;Paludan, Soren R.
通讯作者：
Paludan, Soren R.
影响因子：
3.1
作者：
J. Leu;Shian Jang Yan;Tzuu-fen Lee;Chih Ming Chou;S. Chen;Pung-Pung Hwang-Pung;Chen-Kung Chou;C. Huang
通讯作者：
J. Leu;Shian Jang Yan;Tzuu-fen Lee;Chih Ming Chou;S. Chen;Pung-Pung Hwang-Pung;Chen-Kung Chou;C. Huang