Electric field exposure triggers and guides formation of pseudopod-like blebs in U937 monocytes.
Electric field exposure triggers and guides formation of pseudopod-like blebs in U937 monocytes.
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DOI：
10.1007/s00232-012-9433-7
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发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
Pakhomov AG
中科院分区：
文献类型：
--
作者：
Rassokhin MA;Pakhomov AG
我们描述了纳秒脉冲电场（nsPEF）刺激的 U937 细胞中出现阳极性伪足样起泡的新现象。与细胞损伤反应中常见的“规则”圆形气泡不同，伪足状气泡 (PLB) 形成为朝向阳极的纵向膜突起。在暴露于 10-20 Hz 的 60 ns、10 kV/cm 脉冲下 2 分钟内，PLB 长度可能超过细胞直径。 PLB 和圆形 nsPEF 诱导的气泡都可以通过用较大的溶质（蔗糖）部分等渗替换 NaCl 来有效抑制，从而表明胶体渗透水吸收是气泡形成的主要驱动力。与圆形气泡相反，PLB 在暴露后几分钟内就会收缩。用 1 nM 肌动蛋白聚合阻断剂细胞松弛素 D 处理的细胞无法形成 PLB，而是产生静止的球形泡，没有伸长或收缩能力。活细胞荧光肌动蛋白标记显示，在伸长过程中，肌动蛋白迅速进入 PLB 内部，形成泡皮质和支架，这在静止泡中是看不到的。总体而言，PLB 的形成受活细胞中膜透化的被动（物理化学）效应和主动细胞骨架组装的控制。 PLB在一定程度上模拟了细胞迁移过程中的膜延伸，可以作为细胞力学、膜-细胞骨架相互作用和细胞运动研究的非化学模型。
We describe a new phenomenon of anodotropic pseudopod-like blebbing in U937 cells stimulated by nanosecond pulsed electric field (nsPEF). In contrast to “regular,” round-shaped blebs, which are often seen in response to cell damage, pseudopod-like blebs (PLBs) formed as longitudinal membrane protrusions toward anode. PLB length could exceed the cell diameter in 2 min of exposure to 60-ns, 10-kV/cm pulses delivered at 10–20 Hz. Both PLBs and round-shaped nsPEF-induced blebs could be efficiently inhibited by partial isosmotic replacement of bath NaCl for a larger solute (sucrose), thereby pointing to the colloid-osmotic water uptake as the principal driving force for bleb formation. In contrast to round-shaped blebs, PLBs retracted within several minutes after exposure. Cells treated with 1 nM of the actin polymerization blocker cytochalasin D were unable to form PLBs and instead produced stationary, spherical blebs with no elongation or retraction capacity. Live cell fluorescent actin tagging showed that during elongation actin promptly entered the PLB interior, forming bleb cortex and scaffold, which was not seen in stationary blebs. Overall, PLB formation was governed by both passive (physicochemical) effects of membrane permeabilization and active cytoskeleton assembly in the living cell. To a certain extent, PLB mimics the membrane extension in the process of cell migration and can be employed as a nonchemical model for studies of cytomechanics, membrane–cytoskeleton interaction and cell motility.
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影响因子：
4
作者：
Maugis, Benoit;Brugues, Jan;Amblard, Francois
通讯作者：
Amblard, Francois
DOI：
10.1083/jcb.200802081
发表时间：
2008-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fackler OT;Grosse R
通讯作者：
Grosse R
影响因子：
3.7
作者：
Keller, H;Rentsch, P;Hagmann, J
通讯作者：
Hagmann, J
影响因子：
5
作者：
Andre, Franck M.;Rassokhin, Mikhail A.;Bowman, Angela M.;Pakhomov, Andrei G.
通讯作者：
Pakhomov, Andrei G.
影响因子：
64.8
作者：
Charras, GT;Yarrow, JC;Mitchison, TJ
通讯作者：
Mitchison, TJ