The application of 2H2O to measure skeletal muscle protein synthesis.
The application of 2H2O to measure skeletal muscle protein synthesis.
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DOI：
10.1186/1743-7075-7-31
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发表时间：
2010-04-21
影响因子：
4.5
通讯作者：
Previs SF
中科院分区：
文献类型：
--
作者：
Gasier HG;Fluckey JD;Previs SF
骨骼肌蛋白质合成通常通过使用标记的氨基酸（例如，[13 C]亮氨酸或[13 C]-、[15 N]-或[2 H]苯丙氨酸）作为示踪剂。虽然对于确定蛋白质合成速率是可靠的，但是这种方法学途径需要在受控环境中进行实验，并且因此限制了我们对在长时间自由生活条件下肌肉蛋白质更新的理解（即，综合/累积评估）。另一种示踪剂2 H2O已成功用于测量小鼠、大鼠、鱼类和人类的肌肉蛋白质合成速率。此外，在这些测量中包括了诸如进食和运动等扰动，而不排除常见的环境和生物因素。在这篇综述中，我们讨论了使用2 H2O测量肌肉蛋白质合成的原理，并强调了最近的研究，研究了喂养和运动的影响。在这篇综述中提供的框架应该有助于肌肉生物学家设计实验，以促进我们对骨骼肌改变的条件的理解（例如，运动、进食、生长、衰弱和代谢病变）。
Skeletal muscle protein synthesis has generally been determined by the precursor:product labeling approach using labeled amino acids (e.g., [13C]leucine or [13C]-, [15N]-, or [2H]phenylalanine) as the tracers. Although reliable for determining rates of protein synthesis, this methodological approach requires experiments to be conducted in a controlled environment, and as a result, has limited our understanding of muscle protein renewal under free-living conditions over extended periods of time (i.e., integrative/cumulative assessments). An alternative tracer, 2H2O, has been successfully used to measure rates of muscle protein synthesis in mice, rats, fish and humans. Moreover, perturbations such as feeding and exercise have been included in these measurements without exclusion of common environmental and biological factors. In this review, we discuss the principle behind using 2H2O to measure muscle protein synthesis and highlight recent investigations that have examined the effects of feeding and exercise. The framework provided in this review should assist muscle biologists in designing experiments that advance our understanding of conditions in which anabolism is altered (e.g., exercise, feeding, growth, debilitating and metabolic pathologies).
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DOI：
10.1152/ajpendo.00215.2001
发表时间：
2002-05-01
影响因子：
5.1
作者：
Caso, G;Ford, GC;McNurlan, MA
通讯作者：
McNurlan, MA
DOI：
10.1152/ajpendo.1996.270.2.e313
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影响因子：
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10.1152/ajpendo.00485.2002
发表时间：
2003-05-01
影响因子：
5.1
作者：
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DOI：
10.1016/j.bbagen.2005.12.023
发表时间：
2006-05-01
影响因子：
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