Spontaneous rhythmogenic capabilities of sympathetic neuronal assemblies in the rat spinal cord slice.
Spontaneous rhythmogenic capabilities of sympathetic neuronal assemblies in the rat spinal cord slice.
复制标题
DOI：
10.1016/j.neuroscience.2010.07.007
复制
发表时间：
2010-10-27
期刊：
影响因子：
3.3
通讯作者：
Deuchars, S. A.
中科院分区：
文献类型：
--
作者：
Pierce, M. L.;Deuchars, J.;Deuchars, S. A.
产生节律活动的神经元网络是整个神经系统的共同特征。一些负责节奏行为，如脊髓运动网络;然而，对于其他人来说，功能更微妙，通常涉及信息处理和/或传递。后者的一个例子是交感神经活动，它在体内同步成节律性爆发。与紧张性神经活动相比，这种布置被假定为提供对靶器官反应的改进的控制。传统上，脑干中的神经元回路被认为是产生这些节律的原因，尽管有证据表明，在脊髓化的准备过程中，某些频率是持续存在的。在这里，我们表明，有节奏的人口活动可以记录从中间外侧细胞柱（IML）的胸脊髓切片。记录在10至12日龄大鼠的切片中，这种活动表现为场电位中的8-22 Hz振荡，并且在空间上局限于IML。振荡通常自发发生，但也可通过应用5-HT、α-甲基5-HT或MK212诱导。这些试剂也显着增加自发振荡的强度。节律性活动被TTX消除，并通过应用间隙连接阻断剂或GABAA受体拮抗剂减弱。总之，这些数据表明，这种节奏是一个新兴的特点，脊髓神经元的人口耦合间隙连接。这项工作的假设，交感神经节律依赖于脊髓上的起搏器电路的问题，突出了一个令人惊讶的强大的节律能力的减少交感神经网络的脊髓切片。
Neuronal networks generating rhythmic activity as an emergent property are common throughout the nervous system. Some are responsible for rhythmic behaviours, as is the case for the spinal cord locomotor networks; however, for others the function is more subtle and usually involves information processing and/or transfer. An example of the latter is sympathetic nerve activity, which is synchronized into rhythmic bursts in vivo. This arrangement is postulated to offer improved control of target organ responses compared to tonic nerve activity. Traditionally, oscillogenic circuits in the brainstem are credited with generating these rhythms, despite evidence for the persistence of some frequencies in spinalized preparations. Here, we show that rhythmic population activity can be recorded from the intermediolateral cell column (IML) of thoracic spinal cord slices. Recorded in slices from 10- to 12-day-old rats, this activity was manifest as 8–22 Hz oscillations in the field potential and was spatially restricted to the IML. Oscillations often occurred spontaneously, but could also be induced by application of 5-HT, α-methyl 5-HT or MK212. These agents also significantly increased the strength of spontaneous oscillations. Rhythmic activity was abolished by TTX and attenuated by application of gap junction blockers or by antagonists of GABAA receptors. Together these data indicate that this rhythm is an emergent feature of a population of spinal neurons coupled by gap junctions. This work questions the assumption that sympathetic rhythms are dependent on supraspinal pacemaker circuits, by highlighting a surprisingly strong rhythmogenic capability of the reduced sympathetic networks of the spinal cord slice.
登录
查看更多内容
影响因子：
64.8
作者：
COBB, SR;BUHL, EH;SOMOGYI, P
通讯作者：
SOMOGYI, P
DOI：
10.1111/j.1440-1681.1998.tb02236.x
发表时间：
1998-06-01
影响因子：
2.9
作者：
Kishi, E;Ootsuka, Y;Terui, N
通讯作者：
Terui, N
影响因子：
7.3
作者：
Coker, SJ;Batey, AJ;Eisner, DA
通讯作者：
Eisner, DA
DOI：
10.1073/pnas.0402044101
发表时间：
2004-08-17
影响因子：
11.1
作者：
Cruikshank, SJ;Hopperstadt, M;Srinivas, M
通讯作者：
Srinivas, M
影响因子：
--
作者：
COHEN, MI;GOOTMAN, PM
通讯作者：
GOOTMAN, PM