The biofilm of Burkholderia cenocepacia H111 contains an exopolysaccharide composed of l-rhamnose and l-mannose: Structural characterization and molecular modelling.
The biofilm of Burkholderia cenocepacia H111 contains an exopolysaccharide composed of l-rhamnose and l-mannose: Structural characterization and molecular modelling.
复制标题
DOI：
10.1016/j.carres.2020.108231
复制
发表时间：
2021-01
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
--
作者：
洋葱伯克霍尔德氏菌属于洋葱伯克霍尔德氏菌复合体，由22个临床和环境来源的密切相关物种组成，感染囊性纤维化患者。铜绿假单胞菌占临床分离株的大多数，包括最强毒力和最具传播性的菌株。形成生物膜的能力是盲肠假单胞菌的许多毒力决定因素之一，这一特征使其对一些抗生素、干燥、氧化剂和宿主防御具有更强的耐受性。胞外多糖是生物膜基质的主要成分，特别是为生物膜提供机械稳定性。最近，对阴沟杆菌H111在生物膜中产生的一种不溶于水的胞外多糖进行了鉴定。本研究从盲肠拟青霉H111生物膜中提取了一种水溶性胞外多糖，其结构经GLC-MS、核磁共振和电喷雾质谱确证。重复单元是线性鼠李糖，用α-3-L-曼50%取代3-α-L-Rha。分子模型被用来获得关于局部结构基序的信息，这些信息可以提供关于多糖构象的信息。
Burkholderia cenocepacia belongs to the Burkholderia Cepacia Complex, a group of 22 closely related species both of clinical and environmental origin, infecting cystic fibrosis patients. B. cenocepacia accounts for the majority of the clinical isolates, comprising the most virulent and transmissible strains. The capacity to form biofilms is among the many virulence determinants of B. cenocepacia, a characteristic that confers enhanced tolerance to some antibiotics, desiccation, oxidizing agents, and host defenses. Exopolysaccharides are a major component of biofilm matrices, particularly providing mechanical stability to biofilms. Recently, a water-insoluble exopolysaccharide produced by B. cenocepacia H111 in biofilm was characterized. In the present study, a water-soluble exopolysaccharide was extracted from B. cenocepacia H111 biofilm, and its structure was determined by GLC-MS, NMR and ESI-MS. The repeating unit is a linear rhamnotetrasaccharide with 50 % replacement of a 3-α-l-Rha with a α-3-l-Man. Molecular modelling was used to obtain information about local structural motifs which could give information about the polysaccharide conformation.
登录
查看更多内容
影响因子：
7.4
作者：
Kapaev, Roman R.;Toukach, Philip V.
通讯作者：
Toukach, Philip V.
影响因子：
5.8
作者：
Kapaev, Roman R.;Toukach, Philip V.
通讯作者：
Toukach, Philip V.
影响因子：
3.7
作者：
Cuzzi B;Herasimenka Y;Silipo A;Lanzetta R;Liut G;Rizzo R;Cescutti P
通讯作者：
Cescutti P
影响因子：
3.1
作者：
ALBERSHEIM, PETER;NEVINS, DONALD J.;KARR, ARTHUR
通讯作者：
KARR, ARTHUR
影响因子：
3.1
作者：
HARRIS, PJ;HENRY, RJ;STONE, BA
通讯作者：
STONE, BA