Brockarchaeota, a novel archaeal phylum with unique and versatile carbon cycling pathways.
Brockarchaeota, a novel archaeal phylum with unique and versatile carbon cycling pathways.
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DOI：
10.1038/s41467-021-22736-6
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发表时间：
2021-04-23
影响因子：
16.6
通讯作者：
Baker BJ
中科院分区：
文献类型：
--
作者：
De Anda V;Chen LX;Dombrowski N;Hua ZS;Jiang HC;Banfield JF;Li WJ;Baker BJ
地热环境，如温泉和热液喷口，是碳循环的热点，含有许多描述不清的微生物类群。本研究对中国大陆温泉沉积物和加州湾瓜伊马斯盆地深海热液喷口沉积物中的15个古菌宏基因组进行了重组。这些MAG的系统发育分析表明，他们形成一个独特的组内的TACK超门，因此，我们建议他们的分类作为一个新的门，'Brockarchaeota'，命名后托马斯布罗克他的开创性研究温泉。基于MAG序列信息，我们推断某些布罗克古菌能够通过Wood-Ljungdahl途径的四氢叶酸甲基分支和还原甘氨酸途径介导非产甲烷厌氧甲基营养。热液喷口基因型似乎是植物多糖的专性发酵菌，主要依赖于底物水平的磷酸化，因为它们似乎缺乏大多数呼吸复合物。相比之下，温泉谱系有其他途径来增加其ATP产量，包括甲醇和三甲胺的厌氧甲基营养，并可能使用地热衍生的汞，砷或氢。它们的广泛分布和明显的厌氧代谢多样性表明，Brockarchaeota可能占据以前被忽视的角色，在厌氧碳循环。地球化学环境是碳循环的热点。在这里，De Anda等人从陆地和深海地热沉积物中重建了古菌基因组，并提出将这些微生物分类为一个新的门“Brockarchaeota”，具有独特的代谢能力，包括非产甲烷厌氧甲基营养。
Geothermal environments, such as hot springs and hydrothermal vents, are hotspots for carbon cycling and contain many poorly described microbial taxa. Here, we reconstructed 15 archaeal metagenome-assembled genomes (MAGs) from terrestrial hot spring sediments in China and deep-sea hydrothermal vent sediments in Guaymas Basin, Gulf of California. Phylogenetic analyses of these MAGs indicate that they form a distinct group within the TACK superphylum, and thus we propose their classification as a new phylum, ‘Brockarchaeota’, named after Thomas Brock for his seminal research in hot springs. Based on the MAG sequence information, we infer that some Brockarchaeota are uniquely capable of mediating non-methanogenic anaerobic methylotrophy, via the tetrahydrofolate methyl branch of the Wood-Ljungdahl pathway and reductive glycine pathway. The hydrothermal vent genotypes appear to be obligate fermenters of plant-derived polysaccharides that rely mostly on substrate-level phosphorylation, as they seem to lack most respiratory complexes. In contrast, hot spring lineages have alternate pathways to increase their ATP yield, including anaerobic methylotrophy of methanol and trimethylamine, and potentially use geothermally derived mercury, arsenic, or hydrogen. Their broad distribution and their apparent anaerobic metabolic versatility indicate that Brockarchaeota may occupy previously overlooked roles in anaerobic carbon cycling. Geothermal environments are hotspots for carbon cycling. Here, De Anda et al. reconstruct archaeal genomes from terrestrial and deep-sea geothermal sediments, and propose the classification of these microbes as a new phylum, ‘Brockarchaeota’, with unique metabolic capabilities including non-methanogenic anaerobic methylotrophy.
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影响因子：
16.6
作者：
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通讯作者：
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DOI：
10.1073/pnas.2001063117
发表时间：
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