Overexpression of gasdermin D promotes invasion of adenoid cystic carcinoma.
Overexpression of gasdermin D promotes invasion of adenoid cystic carcinoma.
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Gasdermin D 的过度表达促进腺样囊性癌的侵袭。
DOI：
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发表时间：
2020-07
影响因子：
1.4
通讯作者：
Zhiquan Huang
中科院分区：
文献类型：
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作者：
Ximing Shen;Qianyu Zhang;Zhanghai He;Songhua Xiao;Haigang Li;Zhiquan Huang
目的：研究gasdermin D（GSDMD）表达与腺样囊性癌（ACC）侵袭性的关系。方法：免疫组织化学（IHC）检测GSDMD在33例涎腺ACC患者的肿瘤和邻近非癌组织（ANC）以及29例多形性腺瘤（PA）患者的肿瘤样本中的表达。慢病毒感染用于在ACC-LM和ACC-83细胞（GSDMD-〇 v细胞）中稳定过表达GSDMD，将其进行transwell和划痕试验以评估其与对照细胞相比的侵袭能力。用靶向GSDMD的siRNA处理过表达GSDMD的细胞，随后检查其侵袭能力。结果：GSDMD在ACC组织中的表达显著高于相应的ANC组织（P<0.001）。24小时后，ACC-83和ACC-LM GSDMD-ov细胞系均具有比对照细胞更多的穿过膜的细胞（P<0.05）。对于伤口愈合实验，24小时后GSDMD-〇 v细胞系中的伤口直径小于对照细胞的伤口直径（P<0.001）。高表达GSDMD的ACC细胞系显示出比对照更强的转移能力。结论：GSDMD在ACC组织中的表达高于ANC组织，GSDMD的高表达促进ACC细胞的侵袭。这些结果表明，GSDMD的表达与ACC的侵袭有关。我们的数据表明，我们可能能够使用GSDMD作为肿瘤细胞的侵袭或转移潜力的指标在未来的研究。
OBJECTIVE.To investigate the relationship between gasdermin D (GSDMD) expression and the invasion of adenoid cystic carcinoma (ACC)..METHODS.Immunohistochemistry (IHC) was used to examine GSDMD expression in tumours and adjacent non-cancerous (ANC) tissues from 33 patients with salivary ACC patients and in tumour samples from 29 patients with pleomorphic adenoma (PA). Lentiviral infection was used to stably overexpress GSDMD in ACC-LM and ACC-83 cells (GSDMD-ov cells), which were subjected to transwell and scratch tests to assess their invasive abilities compared to control cells. Cells overexpressing GSDMD were treated with siRNA targeting GSDMD, and their invasive ability was subsequently examined..RESULTS.GSDMD expression was significantly higher in ACC tissues than in corresponding ANC tissues (P<0.001). After 24 hours, both the ACC-83 and ACC-LM GSDMD-ov cell lines had more cells that moved through the membrane than did the control cells (P<0.05). For the wound healing experiment, the diameter of the wound in the GSDMD-ov cell lines was smaller than that of the control cells (P<0.001) after 24 hours. The ACC cell lines expressing high GSDMD showed stronger metastatic ability than did the control..CONCLUSION.GSDMD was highly expressed in ACC tissues compared to ANC tissues, and high GSDMD expression promoted the invasion of ACC cells. These findings suggest that GSDMD expression is related to the invasion of ACC. Our data indicate that we may be able to use GSDMD as an indicator of the invasive or metastatic potential of tumour cells in future research.
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影响因子：
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影响因子：
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