Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos.
Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos.
复制标题
培养系统和蛋白质补充对植入前牛胚胎 mRNA 表达的影响。
作者：
C. Wrenzycki;D. Herrmann;L. Keskintepe;A. Martins;S. Sirisathien;B. Brackett;H. Niemann
在过去的15年里，辅助生殖技术在包括人类在内的大多数哺乳动物物种中取得了巨大进展。越来越多的证据表明，牛植入前发育是一个上级模型，用于研究人类早期发育比小鼠。本研究的目的是探讨两种基本培养系统[含5%CO2的空气中的组织培养液（TCM）或含7%O2、88%N2、5%CO2的合成输卵管液（SOF）]和各种蛋白质补充物的效果（血清，牛血清白蛋白或聚乙烯醇）牛桑椹胚和囊胚中一组发育重要基因转录本的相对丰度，并将结果与体内衍生的对应物进行比较。基本的培养系统，包括基本培养基组成和氧张力有深远的影响，在牛胚胎中的特定转录本的量，而“蛋白质源”只有微弱的影响。在体内和体外发育的胚胎中，特别是在桑椹胚阶段，特异性基因转录本的相对丰度存在显著差异（P <或= 0.05）。TCM系统产生的胚胎与体内衍生胚胎之间的差异大于SOFC产生的胚胎与体内衍生胚胎之间的差异。在两个不同的实验室中，在化学定义的条件下产生的胚胎中基因转录物的相对丰度没有差异。可以得出结论，SOF系统提供了一个环境，其中牛胚胎的植入前发育更类似于发生在体内比在TCM系统。
Assisted reproduction technologies have made great progress during the last 15 years in most mammalian species, including humans. Growing evidence indicates that bovine pre-implantation development is a superior model for investigating early human development than the mouse. The purpose of this study was to investigate the effects of two basic culture systems [tissue culture medium (TCM) with 5% CO(2) in air or synthetic oviduct fluid (SOF) with 7% O(2), 88% N(2,) 5% CO(2)] and various protein supplements (serum, bovine serum albumin or polyvinyl alcohol) on the relative abundance of a set of developmentally important gene transcripts in bovine morulae and blastocysts and to compare the results with those for their in-vivo-derived counterparts. The basic culture system including the basic medium composition and oxygen tension had profound effects on the amounts of specific transcripts in bovine embryos, whereas the 'protein source' had only weak effects. Significant differences (P < or = 0.05) in the relative abundance of specific gene transcripts were detected between in-vivo and in-vitro-derived embryos, especially at the morula stage. More differences were found between embryos produced in the TCM system and in-vivo-derived embryos than between SOF-generated embryos and their in-vivo counterparts. No differences were found in the relative abundance of gene transcripts in embryos generated under chemically defined conditions in the two different laboratories. It is concluded that the SOF system provides an environment in which pre-implantation development of bovine embryos is more similar to that occurring in vivo than in the TCM system.
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影响因子：
13.3
作者：
Bavister, BD
通讯作者：
Bavister, BD
小鼠卵子受精后母体 mRNA 的瞬时多聚腺苷酸化。
DOI：
10.1530/jrf.0.1090223
发表时间：
1997
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Temeles,GL;Schultz,RM
通讯作者：
Schultz,RM
影响因子：
--
作者：
WILLOTT, E;BALDA, MS;ANDERSON, JM
通讯作者：
ANDERSON, JM
DOI：
10.1530/jrf.0.0990673
发表时间：
1993-11-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
FISCHER, B;BAVISTER, BD
通讯作者：
BAVISTER, BD