Microfluidic lab-on-a-chip derivatization for gaseous carbonyl analysis.
Microfluidic lab-on-a-chip derivatization for gaseous carbonyl analysis.
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用于气态羰基分析的微流控芯片实验室衍生化。
DOI：
10.1016/j.chroma.2013.04.066
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Pang X
中科院分区：
文献类型：
--
作者：
Pang X
提出了一种以O-（2，3，4，5，6-五氟苄基）羟胺（PFBHA）为衍生试剂的微流控芯片实验室衍生化技术，用于气态羰基化合物的分析。新型微流控芯片实验室衍生化技术已被开发用于测量气态羰基化合物的nmol/mol（ppbv）混合比，这对大气化学特别重要。该技术使用了一个平面玻璃微反应器，包括三个入口和一个出口，气体和流体分离和组合通道，混合接头，和一个2.0米长，620μm内径的反应微通道。微反应器集成了三个功能，提供：（1）气体和液体混合器和反应器，（2）试剂加热，和（3）样品预浓缩。衍生化溶液的浓度、进入的气体样品和PFBHA溶液的体积流速以及微反应器的温度被优化以实现近实时测量。微反应器中增强的相接触面积与体积比和高传热速率导致快速高效的衍生化反应，产生准备直接引入GC-MS的流出物流。在最佳反应条件下，对8种羰基化合物进行衍生化，在1- 500 ppbv范围内线性关系良好。在这项研究中，大多数羰基化合物的方法检测限（MDLs）低于0.10nmolmol− 1，低于或接近其在清洁环境空气中的典型浓度。该技术的性能进行了评估，通过应用该方法的量化乙二醛（GLY）和甲基乙二醛（MGLY）在异戊二烯光氧化过程中形成的室外光反应室（EUPHORE）。GLY和MGLY测量之间的良好协议，获得比较这种新技术与傅里叶变换红外光谱（FTIR），这提供了支持的微流体技术的气体测量的潜在有效性。
We present a microfluidic lab-on-a-chip derivatization technique for the analysis of gaseous carbonyl compounds using O-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine (PFBHA) as the derivatizing reagent. The novel microfluidic lab-on-a-chip derivatization technique has been developed to measure nmol per mole (ppbv) mixing ratios of gaseous carbonyl compounds, which are of particular importance to atmospheric chemistry. The technique utilised a planar glass microreactor comprising three inlets and one outlet, gas and fluid splitting and combining channels, mixing junctions, and a 2.0m long, 620μm internal diameter reaction microchannel. The microreactor integrated three functions, providing: (1) a gas and liquid mixer and reactor, (2) reagent heating, and (3) sample pre-concentration. The concentration of derivatization solution, the volumetric flow rates of the incoming gas sample and PFBHA solution, and the temperature of the microreactor were optimised to achieve a near real-time measurement. The enhanced phase contact area-to-volume ratio and the high heat transfer rate in the microreactor resulted in a fast and high efficiency derivatization reaction, generating an effluent stream which was ready for direct introduction to GC–MS. Good linearity was observed for eight carbonyl compounds over the measurement ranges of 1–500ppbv when they were derivatized under optimal reaction conditions. The method detection limits (MDLs) were below 0.10nmolmol−1for most carbonyls in this study, which is below or close to their typical concentrations in clean ambient air. The performance of the technique was assessed by applying the methodology to the quantification of glyoxal (GLY) and methylglyoxal (MGLY) formed during isoprene photo-oxidation in an outdoor photoreactor chamber (EUPHORE). Good agreements between GLY and MGLY measurements were obtained comparing this new technique with Fourier Transform InfraRed (FTIR), which provides support for the potential effectiveness of the microfluidic technique for gaseous measurements.
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DOI：
10.1073/pnas.93.18.9821
发表时间：
1996-09-03
影响因子：
11.1
作者：
Herman, JG;Graff, JR;Baylin, SB
通讯作者：
Baylin, SB
DOI：
10.1039/b513999f
发表时间：
2006-03
期刊：
The Analyst
影响因子：
--
作者：
Y. Takabayashi;M. Uemoto;Kenjiro Aoki;T. Odake;T. Korenaga
通讯作者：
Y. Takabayashi;M. Uemoto;Kenjiro Aoki;T. Odake;T. Korenaga
DOI：
--
发表时间：
1991
期刊：
影响因子：
--
作者：
W. Ligon;H. Grade
通讯作者：
H. Grade
3-甲基-2-苯并噻唑酮腙法在大气分析脂肪醛中的应用。
DOI：
--
发表时间：
1963
期刊：
影响因子：
--
作者：
A. P. Altshuller;L. Leng
通讯作者：
L. Leng
影响因子：
4.1
作者：
Lewis, Alastair C.;Hamilton, Jacqueline F.;Milton, Martin J. T.
通讯作者：
Milton, Martin J. T.