Bacterial ghost of avian pathogenic E. coli (APEC) serotype O78:K80 as a homologous vaccine against avian colibacillosis.
Bacterial ghost of avian pathogenic E. coli (APEC) serotype O78:K80 as a homologous vaccine against avian colibacillosis.
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DOI：
10.1371/journal.pone.0194888
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Khan MI
中科院分区：
文献类型：
--
作者：
Ebrahimi-Nik H;Bassami MR;Mohri M;Rad M;Khan MI
禽大肠杆菌病是全世界家禽业经济损失的主要原因之一，对发展中国家的影响更为明显。几种细菌的参与使得开发针对这种疾病的有效疫苗具有挑战性，特别是因为众所周知，很难制造包含所有致病细菌的疫苗。在这里，我们报告的设计和制造的细菌鬼（BG）的E。coliO78：K80是引起该病的主要细菌血清型之一。产生的鬼，然后利用作为同源疫苗对禽大肠杆菌病。我们证明了E. coliO78：K80在光密度高达0.8时也能正常发生，而大肠杆菌等细菌的光密度为0.3 - 0.4。coli TOP 10。这对于大规模生产这种鬼是特别有利的，这是开发任何基于BG的疫苗的重要因素。与E. coliTOP10中，我们在用E-裂解质粒转化野生型细菌时面临很大的挑战，这可能是由于O78：K80的细胞壁更厚。通过注射以及通过呼吸系统将疫苗以1010 BG的剂量给予Ross 308肉鸡，以每周间隔重复3次。然后在最后一次免疫后一周用剂量为1011个细菌的野生型O78：K80与传染性支气管炎H120疫苗（作为免疫抑制剂）一起攻击鸡。与对照组相比，BG接种组的气囊病变显著减少。测定免疫鸡血清中IFN γ、IgA和IgY的水平作为免疫应答的指标，并与灭活菌免疫鸡进行比较。结果表明，O78：K80 BG可作为禽大肠杆菌病的有效同源疫苗。我们希望我们的研究结果可以作为设计更复杂的疫苗的起点，这些疫苗包含与禽大肠杆菌病有关的所有三种主要致病菌。
Avian Colibacillosis is among the major causes of economic loss in the poultry industry worldwide, with a more vivid impact on developing countries. The involvement of several bacteria has made it challenging to develop effective vaccines for this disease, particularly because it is notoriously difficult to make a vaccine that contains all the contributing pathogenic bacteria. Here, we report the design and fabrication of a bacterial ghost (BG) of E. coli O78:K80, which is among the major bacterial serotypes responsible for this disease. The generated ghost is then exploited as a homologous vaccine against Avian Colibacillosis. We demonstrate that hole formation in the cell wall of E. coli O78:K80 can happen properly in optical densities as high as 0.8 compared to the 0.3–0.4 standard for bacteria like E. coli TOP10. This is especially advantageous for mass production of this ghost which is a vital factor in development of any BG-based vaccine. Compared to E. coli TOP10, we faced a great challenge in transforming the wild type bacteria with the E-lysis plasmid which was probably due to higher thickness of the cell wall in O78:K80. This, however, was addressed by treating the cell wall with a different combination of ions.The vaccine was administered to Ross 308 broiler chickens via injection as well as through their respiratory system at a dose of 1010 BGs, repeated 3 times at weekly intervals. Chickens were then challenged with the wild type O78:K80 at a dose of 1011 bacteria together with Infectious Bronchitis H120 vaccine (as immunosuppressant) one week after the last immunization. Air sac lesions were significantly reduced in BG vaccinated groups in comparison with the control group. The levels of IFNγ, IgA and IgY were measured in the serum of immunized chickens as an indication of immune response and were compared with those of the chickens vaccinated with killed bacteria. The results show that O78:K80 BG can be used as an efficient homologous vaccine against Colibacillosis disease in poultry. We expect our findings can serve as the starting point for designing more sophisticated vaccines that contain all three major pathogenic bacteria involved in avian Colibacillosis.
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DOI：
10.3390/ijerph7010089
发表时间：
2010-01
影响因子：
--
作者：
Lutful Kabir SM
通讯作者：
Lutful Kabir SM
影响因子：
2.4
作者：
Derakhshandeh, A.;Salehi, T. Zahraei;Karimi, V.
通讯作者：
Karimi, V.
DOI：
10.1016/b978-0-12-800879-9.00049-4
发表时间：
2017-01-01
期刊：
EGG INNOVATIONS AND STRATEGIES FOR IMPROVEMENTS
影响因子：
--
作者：
Kabir, S. M. Lutful;Sikder, Mahmudul Hasan;Yamasaki, Shinji
通讯作者：
Yamasaki, Shinji
影响因子：
1.4
作者：
KLEVEN, SH;KING, DD;ANDERSON, DP
通讯作者：
ANDERSON, DP
影响因子：
4.9
作者：
Charleston, B;Gate, JJ;Froyman, R
通讯作者：
Froyman, R