Trichostatin A selectively suppresses the cold-induced transcription of the ZmDREB1 gene in maize.
Trichostatin A selectively suppresses the cold-induced transcription of the ZmDREB1 gene in maize.
复制标题
曲古抑菌素 A 选择性抑制玉米中 ZmDREB1 基因的冷诱导转录
DOI：
10.1371/journal.pone.0022132
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Hu Y;Zhang L;Zhao L;Li J;He S;Zhou K;Yang F;Huang M;Jiang L;Li L
组蛋白的翻译后修饰在应对环境压力方面起着至关重要的作用。组蛋白脱乙酰酶(HDAC)催化从组蛋白中去除乙酰基，通常被认为是一种转录抑制因子。在这篇文章中，我们报告了冷处理高度诱导HDACs的上调，导致组蛋白H3和H4的整体脱乙酰化。在冷胁迫条件下，用HDAC抑制剂曲古抑素A(TSA)处理玉米，强烈地抑制了玉米冷响应基因ZmDREB1和ZmCOR413的诱导。然而，冷应激对ZmICE1基因的上调影响较小。干旱和盐诱导基因ZmDBF1和rab17的表达几乎不受TSA处理的影响。因此，这些观察结果表明，HDAC可以选择性地激活转录。TSA对ZmDREB1和ZmCOR413基因表达的影响的时间进程表明，HDAC似乎直接激活ZmDREB1基因，进而调节ZmCOR413的表达。冷处理后，ICE1结合区发生组蛋白超乙酰化和DNA去甲基化，同时微球菌核酸酶(MNase)的可及性增加。与ICE1结合位点相邻的两个区域仍处于低乙酰化和甲基化状态。然而，在冷习服期间，TSA处理增加了MNase的乙酰化状态和可及性，并降低了这两个区域的DNA甲基化。然而，TSA治疗并不影响ZmDREB1基因ICE1结合区的组蛋白超乙酰化和DNA甲基化水平。综上所述，我们的发现表明，HDAC通过组蛋白修饰和染色质构象变化来正向调节冷诱导的ZmDREB1基因的表达，这种激活既是基因选择性的，也是位置选择性的。
Post-translational modifications of histone proteins play a crucial role in responding to environmental stresses. Histone deacetylases (HDACs) catalyze the removal of an acetyl group from histones and are generally believed to be a transcriptional repressor. In this paper, we report that cold treatment highly induces the up-regulation of HDACs, leading to global deacetylation of histones H3 and H4. Treatment of maize with the HDAC inhibitor trichostatin A (TSA) under cold stress conditions strongly inhibits induction of the maize cold-responsive genes ZmDREB1 and ZmCOR413. However, up-regulation of the ZmICE1 gene in response to cold stress is less affected. The expression of drought and salt induced genes, ZmDBF1 and rab17, is almost unaffected by TSA treatment. Thus, these observations show that HDACs may selectively activate transcription. The time course of TSA effects on the expression of ZmDREB1 and ZmCOR413 genes indicates that HDACs appear to directly activate the ZmDREB1 gene, which in turn modulates ZmCOR413 expression. After cold treatment, histone hyperacetylation and DNA demethylation occurs in the ICE1 binding region, accompanied by an increase in accessibility to micrococcal nuclease (MNase). The two regions adjacent to the ICE1 binding site remain hypoacetylated and methylated. However, during cold acclimation, TSA treatment increases the acetylation status and accessibility of MNase and decreases DNA methylation at these two regions. However, TSA treatment does not affect histone hyperacetylation and DNA methylation levels at the ICE1 binding regions of the ZmDREB1 gene. Altogether, our findings indicate that HDACs positively regulate the expression of the cold-induced ZmDREB1 gene through histone modification and chromatin conformational changes and that this activation is both gene and site selective.
登录
查看更多内容
影响因子：
7.2
作者：
Kizis, D;Pagès, M
通讯作者：
Pagès, M
影响因子：
4.9
作者：
Qin, F;Sakuma, Y;Yamagushi-Shinozaki, KY
通讯作者：
Yamagushi-Shinozaki, KY
影响因子：
7.2
作者：
Li, Y;Butenko, Y;Grafi, G
通讯作者：
Grafi, G
影响因子：
7.2
作者：
Niogret, MF;CulianezMacia, FA;Pages, M
通讯作者：
Pages, M
影响因子：
5.1
作者：
Haring M;Offermann S;Danker T;Horst I;Peterhansel C;Stam M
通讯作者：
Stam M